Anti-mitochondrial antibodies in patients with primary biliary cirrhosis detected by double immunodiffusion and enzyme-linked immunosorbent assay using mitochondria antigens obtained from rat liver and bovine heart.

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A high incidence of antibody against mitochondrial antigen (AMA) has been demonstrated in sera of patients with primary biliary cirrhosis (PBC). AMA has usually been detected by the indirect immunofluorescence (IF) test using tissue sections, however this method has no specificity. In this study the sera of patients with PBC, along with the other liver diseases and collagen diseases, were examined for the antibodies against mitochondria antigens obtained from bovine heart and rat liver by means of double immunodiffusion (DID) and enzyme-linked immunosorbent assay (ELISA) in order to investigate the diversity and the specificity of AMA. In DID using bovine heart mitochondria, three precipitating antibodies were detected and referred to Fa, Fb and Fc antigen. The incidence of antibodies in PBC to these three antigens were 100%, 76% and 7% respectively. Three antibodies were detected only in PBC, but not in other liver diseases, collagen diseases nor healthy blood donor sera. On the other hand, in DID using rat liver mitochondria, the antibody to Fb antigen alone was detected in 12 out of 17 PBC sera but the antibody to Fa antigen was not detected. In ELISA using bovine heart mitochondria, high titers of AMA ranging from 1: 320 to 1: 10, 240 were recognized in 13 PBC sera which have precipitating antibodies to both Fa and Fb antigen. On the other hand, low titers of AMA or negative reaction were obtained in 4 PBC sera which have antibody to Fa antigen alone. These results would indicate a polymorphism of mitochondrial antigen among the tissues of the different animals.
社団法人日本肝臓学会の論文

Anti-mitochondrial antibodies in patients with primary biliary cirrhosis detected by double immunodiffusion and enzyme-linked immunosorbent assay using mitochondria antigens obtained from rat liver and bovine heart.

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