急性肝不全に対するglucagon-insulin療法に関する研究-2-基礎的研究

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Our previous reports have made clear that glucagon and insulin stimulate the liver regeneration by their synergistic action in experimental and clinical trials. In this paper we aimed to reveal interaction of their pancreatic hormones to molecular levels in the damaged liver. Rats were administered of d-galactosamine (1000mg/kg body weight) by intraperitoneal injection. After 24 hours we treated with administration of glucagon (1mg/kg body weight) and regular insulin (1unit/kg body weight). These rats were divided into three groups as the followes; glucagon-insulin group, glucagon group and control group. After studying of the biological parameters for the liver regeneration, we revealed positively the localization of alpha-fetoprotein, cyclic AMP by immunofluorescence method in liver tissue and DNA synthesis in vivo by autoradiography of 3H-thymidine. Survivors were significantly enhanced with administration of glucagon and insulin in comparison to glucagon alone and control. It is concluded that administration of glucagon and insulin simultaneously stimulate the biological parameters and improve the survival rate.
社団法人日本肝臓学会の論文