OXIDOREDUCTASES AND SUGAR METABOLISMS OF SACCHAROMYCES CEREVISIAE GROWN ON ETHANOL

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1. In the growth medium containing ethanol as a sole carbon source, a strain of S. cerevisiae did not multiply but its respiratory activity was fully developed. Oxidoreductases and sugar metabolism of yeast cells grown on glucose and on ethanol were investigated.2. Cytochrome oxidase activity and the amount of ubiquinone were much higher in the ethanol-grown cells than in the glucose-grown cells. The amount of total flavin was idenentical in both cells.3. The development of the respiratory activity during the cultivation in the ethanol-medium was inhibited by chloramphenicol and Actinomycin D, but not by Mitomycin C. Cells cultivated in the presence of spermine showed somewhat inhibited oxygen uptake. But it is not clear at present that it is due to the inhibition of the development of respiratory activity or merely to the inhibitory effect of spermine on respiration.4. The amounts of trehalose and mannan were identical in both the glucosegrown and the ethanol-grown cells, but glycogen was much higher in the glucosegrown cells.5. The activities of phosphorylase, glucose-6-phosphate dehydrogenase and phosphogluconate dehydrogenase were identical in both cells. The activities of glucose-6-phosphatase, ribosephosphate isomerase, lactate dehydrogenase and isocitrate: NADP oxidoreductase were higher in the ethanol-grown cells than in the glucose-grown cells.
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