Mechanism of augmentation of natural cytotoxicity by hyman beta-interferon.
スポンサーリンク
概要
- 論文の詳細を見る
Natural killer (NK) cells have been demonstrated to be activated by interferon (IFN). However the mechanisms of this activation are still not well known. The aim of this study is to investigate the mechanisms of NKstimulation by IFN against human leukemia cell line K562, human renal cell carcinoma cell line CaKi 1, and human prostatic carcinoma cell line DU145, using the methods of 51Cr-release assay and single cell cytotoxicity assay.From the results of single cell cytotoxicity assay, frequency of active NK cells among peripheral blood lymphocytes (PBL) against each cell line increased significantly by preincubation of PBL with 2, 000 IU/ml of human beta interferon (HuIFN-β) for 12 hr. Recycling capacity of NK cells was measured combining single cell cytotoxicity assay and 3 hr 51Cr-release assay, using K562 cells as target cells. By this measurement maximal recycling capacity (MRC) increased from 10.9±2.2 to 17.8±2.9 (n=7, p<0.01).It was concluded that stimulation of PBL with HuIFN-β resulted in increase of active NK cells and recycling capacity, i.e. function of individual NK cells to recycle after killing of the initially encountered tumor cells.
- The Keio Journal of Medicineの論文
著者
-
Hayakawa Masamichi
Department Of Chemistry Faculty Of Science Gakushuin University
-
Marumo Ken
Department Of Urology Keio University School Of Medicine
-
Ueno Munehisa
Department Of Urology Biomedical Research Center Saitama Medical School
-
Tazaki Hiroshi
Department Of Urology Keio University School Of Medicine
-
Murai Masaru
Department Of Public Health Sapporo Medical University School Of Medicine
-
UENO MUNEHISA
Department of Urology, School of Medicine, Keio University
-
JITSUKAWA SEIDO
Department of Urology, School o f Medicine, Keio University
関連論文
- Production of erythropoietin and multiple cytokines by metanephric adenoma results in erythrocytosis
- Prediction of extraprostatic extension by prostate specific antigen velocity, endorectal MRI, and biopsy Gleason score in clinically localized prostate cancer
- W3-5 Laparoscopic radical prostatectomy : extraperitoneal approach(Keynote Lecture,Workshop 3 「Laparoscopic Radical Prostatectomy-Outcome of Each Approach」)
- The value of γ-seminoprotein in combination with prostate specific antigen in detecting prostate cancer
- N-Acetylcysteine Modifies cis-Dichlorodiammineplatinum-induced Effects in Bladder Cancer Cells
- The Clinical Effectiveness of Laser-Lithotripsy for Ureteral Calculi with an Automatic Stone Detection System
- Impact of thrombocytosis and C-reactive protein elevation on the prognosis for patients with renal cell carcinoma
- New genetic causes of pheochromocytoma : current concepts and the clinical relevance
- Transluminal Vascular Stent Implantation for a Patient with Renovascular Hypertension Due to Renal Artery Stenosis
- Long-term results of three different minimally invasive therapies for lower urinary tract symptoms due to benign prostatic hyperplasia : Comparison at a single institute
- Differential Diagnosis of Primary Benign and Malignant Retroperitoneal Tumors
- TREATMENT OF BENIGN PROSTATIC HYPERPLASIA WITH HIGH INTENSITY FOCUSED ULTRASOUND: AN INITIAL CLINICAL TRIAL IN JAPAN WITH MAGNETIC RESONANCE IMAGING OF THE TREATED AREA
- High Intensity Focused Ultrasound for Benign Prostatic Hyperplasia; A 6-month Follow-up
- A NEW SCORING SYSTEM BASED ON THE HISTOLOGICAL BEHAVIOR AND PROLIFERATIVE ACTIVITY OF TUMOR CELLS FOR GRADING THE MALIGNANT POTENTIAL OF BLADDER CANCERS
- Transurethral Microwave Hyperthermia of Benign Prostatic Hyperplasia
- Detection of IL-2 Receptor Gene Expression in Peripheral Blood from Renal Transplant Patients
- The prevalence of renal cell carcinoma : A nation-wide survey in Japan in 1997
- Bilateral adrenal leiomyosarcoma treated with multiple local therapies
- Surgical outcomes of partial nephrectomy for renal cell carcinoma : A joint study by the Japanese Society of Renal Cancer
- Incidentally discovered giant renal arteriovenous malformation
- Adult Wilms' tumor: clinical characteristics of four cases and review of the literature
- Simultaneous Presentation of Malignant Lymphoma of the Kidney and Multiple Myeloma of the Bone Marrow
- Synthesis and Photochemical Reactions of Polystannanes
- Gene Expression of TGF-α, EGF and IL-6 in Cultured Renal Tubular Cells and Renal Cell Carcinoma
- Transurethral needle ablation of the prostate : An initial Japanese clinical trial
- JSSM Guidelines for erectile dysfunction
- A 35-year-old Man with Cerebral Hemorrhage and Pheochromocytoma : The Second Brain-dead Organ Donor in Japan
- Strip biopsy of superficial bladder tumors using an electrosurgical snare
- Establishment of a Human Cell Line Secreting Neuron-specific Enolase from a Primitive Neuroectodermal Tumor of the Retroperitoneal Cavity
- Maximum tumor diameter is a simple and valuable index associated with the local extent of disease in clinically localized prostate cancer
- Three-dimensional ultrasonography in evaluation of benign prostatic hyperplasia
- Incidence and growth pattern of simple cysts of the kidney in patients with asymptomatic microscopic hematuria
- Significance and diagnostic accuracy of renal calculi found by ultrasonography in patients with asymptomatic microscopic hematuria
- What are the Contents of a Hydrocele Testis?
- Massive Urinary Extravasation from the Renal Pelvis following Ileal Loop Diversion
- Primary carcinoid tumor in a polycystic kidney
- Expression of Ets-1 in human clear cell renal cell carcinomas : Implications for angiogenesis
- Treatment efficiency of a suicide gene therapy using prostate-specific membrane antigen promoter/enhancer in a castrated mouse model of prostate cancer
- Development of Gene Therapy Using Prostate-specific Membrane Antigen Promoter/Enhancer with Cre Recombinase/LoxP System for Prostate Cancer Cells under Androgen Ablation Condition
- Suicide gene therapy on LNCaP human prostate cancer cells
- Effect of herbimycin A on renal cancer cell growth
- Transitional cell carcinoma of ureteral stump after radical nephrectomy for renal cell carcinoma
- Aldosterone-Producing Adenoma with Prolonged Amelioration by Dexamethasone
- Production of parathyroid hormone-related protein in two new cell lines of renal cell carcinoma
- Simultaneous production of granulocyte colonystimulating factor and parathyroid hormone-related protein in bladder cancer
- Suppression of default apoptosis in androgen-dependent cells by testosterone-mediated bcl-2 expression
- Laparoscopic treatment of retroperitoneal benign schwannoma
- A New Technique of Arteriovenous Fistula with Titanium Clip Appller
- Non-functional adrenocortical adenoma with extensive degeneration
- S13-6 Gateways to apoptosis : the role of the cytokine-dependent signal transduction pathways in renal cell carcinoma
- Re-evaluation of the latent period of bladder cancer in dyestuff-plant workers in Japan
- Transurethral Microwave Thermotherapy for Benign Prostatic Hyperplasia; Comparison of Different Treatment Protocols
- CP8-9 NF-_κB function inhibitor, DHMEQ as a treatment option for advanced urological cancer
- An Upregulation of Interleukin-2 Receptor, Transferrin Receptor Expression and Cytokine Production Mediated by Hemin in Human Peripheral Blood Mononuclear Cells
- Dexamethasone and interleukin-2 combination therapy for advanced renal cell carcinoma in a patient with paraneoplastic inflammatory syndrome
- Age-related prevalence of erectile dysfunction in Japan : Assessment by the International Index of Erectile Function
- Nephrotic syndrome as a result of membranous nephropathy caused by renal cell carcinoma
- Effects of quercetin on the heat-induced cytotoxicity of prostate cancer cells
- Characterization of a newly established cell line derived from human adrenocortical carcinoma
- Hypercalcemia in a patient with renal cell carcinoma producing parathyroid hormone-related protein and interleukin-6
- Oncolytic Viral Therapy for Human Prostate Cancer by Conditionally Replicating Herpes Simplex Virus 1 Vector G207
- Usefulness of laparoscopy to find the distal vas deferens during vasovasostomy for a patient with iatrogenic injury of the vas deferens during inguinal herniorrhaphy
- Three-year results of treatment for prostate cancer with low-dose rate temporary iridium-192 brachytherapy
- Brachytherapy for prostate cancer in Japan
- Prostate-specific Membrane Antigen-derived Primers in a Nested Reverse Transcription Polymerase Chain Reaction for Detecting Prostatic Cancer Cells
- Aging and erectile dysfunction : The role of aging and concomitant chronic illness
- Screening of H-ras Gene Point Mutations in 50 Cases of Bladder Carcinoma
- Sex among Asian men and women : The Global Better Sex Survey in Asia
- Severe polyuria after the resection of adrenal pheochromocytoma
- Elevated serum IgG4 may predict sensitivity to steroid therapy in retroperitoneal fibrosis
- Erectile function and nocturnal penile tumescence in patients with prostate cancer undergoing luteinizing hormone-releasing hormone agonist therapy
- Experience with Massive Anti-Cancer Chemotherapy under Hyperbaric Oxygen Pressure in Terminal Urologic Malignancy
- Biochemical Modulation of 5-Fluorouracil with Murine Interferon-α/β Against Murine Renal Cell Carcinoma
- Application of the Interferon Minipellet to Human Renal Cell Carcinoma in Nude Mice
- Antitumor Effects of an Interferon-Loaded Silicone Formulation in Human Renal Cell Carcinoma in Nude Mice
- Pharmacokinetics and Antitumor Effects of an Interleukin-2 Immunocomplexing Agent in Murine Renal Cell Carcinoma
- A case report of retroaortic left renal vein with tumor thrombus of renal cell carcinoma
- How far should we treat metastatic liver cancer? Report of three long-term survivors
- PATHOLOGICAL STUDIES ON THE PROSTATE GLANDS OF JAPANESE, WITH SPECIAL REFERENCE TO LATENT MALIGNANCY
- Significant Correlation between Chromosomal Aberration and Nuclear Morphology in Urothelial Carcinoma
- A FUSED PELVIC KIDNEY
- Latent hypernephroma.
- Ex-vivo surgery for treatment of intrarenal A-V malformation.
- Mechanism of augmentation of natural cytotoxicity by hyman beta-interferon.
- Retrograde brushing to the urothelial tumors of the upper urinary tract for early diagnosis and treatment.
- ORGAN CULTURE OF HUMAN BLADDER TUMORS UNDER HYPERBARIC OXYGEN