Mitogen Induced Cell-mediated Cytotoxicity (MICC) in Human Peripheral Blood Granulocytes and Lymphocytes:I. Possible effector mechanisms

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Mitogen induced cell-mediated cytotoxicity (MICC) of human peripheral blood granulocytes (PMN) and lymphocytes (PBL) were studied by employing chicken erythrocytes as targets. MICC mediated by both effectors revealed similar dose-response and temperature-dependency was only significantly induced by T cell mitogen but not by B cell mitogen. PMN were found to exhibit more effective cytotoxicity than PBL in our study. The direct contact of effectors and targets was essential to induce MICC. Participation of erythrophagocytosis was not supported in MICC activity and supernatant obtained from cultivated effector-target-lectin ligand or soluble sonicated PMN lysate did not affect MICC. Additionally, MICC inhibition study was performed in an attempt to disclose certain possible effector mechanisms of MICC. The results indicated that membrane active agents, anti-tubulin, anti-microfilament, EDTA, local anesthetics and soluble immune complex considerably suppressed the MICC activity. DNA-inhibitor and specific sugar had moderate effect whereas gold salt, insoluble immune complex or membrane Na+-K+-ATPase inhibitor showed little effect on the activity. These observations provided us to conclude that the full expression of MICC required functionally preserved lectin reactive membrane receptors, intact cytoskeletal system, active DNA synthesis and presence of divalent cations.
日本臨床免疫学会の論文