Basic Fibroblast Growth Factor-Like Substance Associates with DNA in Germ Cell Nuclei
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概要
- 論文の詳細を見る
Our previous work has demonstrated that immunoreactive basic fibroblast growth factor (bFGF)-like substance presents in germ cell nuclei of adult rat testis. In the present study, interactions of bFGF-like substance with a sub-uclear material were investigated. When nuclear extract from adult rat germ cell was analysed by heparin-Sepharose affinity chromatography and Western blot, germ cell nuclei contained 2 forms of bFGF-like substances with molecular weights of 16.5 and 24.5 kDa. The immunoreactivity for bFGF-like substance of germ cell nuclei decreased after treatment with either micrococcal nuclease or DNase l. Furthermore, South-Western blots analysis revealed these 2 forms of bFGF-like substance bind with high affinity to the exogenous salmon testis DNA. Thus, these results suggest that the bFGF-like substances are associated with DNA in the germ cell.
- 日本繁殖生物学会の論文
著者
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Kamei Takayuki
Department Of Veterinary Physiology Nippon Veterinary And Animal Science University
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Kamei Takayuki
Department Of Applied Physics Tokyo University Of Science
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Kamei Takayuki
The Department Of Computer Science Keio University:toshiba Corporation
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Suzuki Katsushi
Department Of Veterinary Physiology Nippon Veterinary And Zootechnical College
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Shiota Kunio
Cellular Biochemistry, Animal Resource Sciences, The University of Tokyo
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OGAWA TOMOYA
Cellular Biochemistry, Veterinary Medical Science/Animal Resource Science, University of Tokyo
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TAKAHASHI Michio
Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
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Ogawa Tomoya
Cellular Biochemistry Veterinary Medical Science/animal Resource Science University Of Tokyo
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KAMEI Takayuki
Department of Veterinary Physiology, Nippon Veterinary and Animal Science University
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