Basic Fibroblast Growth Factor-Like Substance Associates with DNA in Germ Cell Nuclei
スポンサーリンク
概要
- 論文の詳細を見る
Our previous work has demonstrated that immunoreactive basic fibroblast growth factor (bFGF)-like substance presents in germ cell nuclei of adult rat testis. In the present study, interactions of bFGF-like substance with a sub-uclear material were investigated. When nuclear extract from adult rat germ cell was analysed by heparin-Sepharose affinity chromatography and Western blot, germ cell nuclei contained 2 forms of bFGF-like substances with molecular weights of 16.5 and 24.5 kDa. The immunoreactivity for bFGF-like substance of germ cell nuclei decreased after treatment with either micrococcal nuclease or DNase l. Furthermore, South-Western blots analysis revealed these 2 forms of bFGF-like substance bind with high affinity to the exogenous salmon testis DNA. Thus, these results suggest that the bFGF-like substances are associated with DNA in the germ cell.
- 日本繁殖生物学会の論文
著者
-
Kamei Takayuki
Department Of Veterinary Physiology Nippon Veterinary And Animal Science University
-
Kamei Takayuki
Department Of Applied Physics Tokyo University Of Science
-
Kamei Takayuki
The Department Of Computer Science Keio University:toshiba Corporation
-
Suzuki Katsushi
Department Of Veterinary Physiology Nippon Veterinary And Zootechnical College
-
Shiota Kunio
Cellular Biochemistry, Animal Resource Sciences, The University of Tokyo
-
OGAWA TOMOYA
Cellular Biochemistry, Veterinary Medical Science/Animal Resource Science, University of Tokyo
-
TAKAHASHI Michio
Veterinary Physiology, Veterinary Medical Science, The University of Tokyo
-
Ogawa Tomoya
Cellular Biochemistry Veterinary Medical Science/animal Resource Science University Of Tokyo
-
KAMEI Takayuki
Department of Veterinary Physiology, Nippon Veterinary and Animal Science University
関連論文
- Generation and Characterization of a Monoclonal Antibody Recognizing a Fetal Brain Enriched O-Linked Sialoglycoprotein, FOG100
- Locus of Dominant Hairless Gene (Ht) Causing Abnormal Hair and Keratinization Maps to Rat Chromosome 10
- Epigenetics in Toxicology Research : Application of Genome-wide DNA Methylation Profiles(Proceedings of the 32nd Annual Meeting)
- Molecular Cloning of Equine Preprorelaxin cDNA
- Influence of the hypogonadism (hgn) locus on female reproduction and ovarian development in an altered genetic background
- Teratogenic effect of static magnetic field on mouse fetuses. (DEVELOPMENT AND REPRODUCTION) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th Annual Meeting)
- Developmental disruption by bisphenol A in early chick embryos. (DEVELOPMENT AND REPRODUCTION) (GENERAL SESSION BY POSTER PRESENTATION) (Proceedings of the 30th Annual Meeting)
- Prolactin Receptor mRNA Expression in Fetal Rat Brain
- Expression of LH-α and -β Subunit mRNAs in the Rat Placenta
- Postnatal Alterations of Testicular Morphology, Testicular Testosterone Contents and Plasma Hormone Levels in Male Hypogonadic Rat (hgn/hgn)
- Reduced Fertility in Female Homozygotes for hgn (Male Hypogonadism) Selected by hgn-Associated Hypoplastic Kidney
- Genetic Analysis and Histology of Hypoplastic Kidneys in the Male Hypogonadic Mutant (hgn/hgn) Rat
- Study on Cause of Death in Rat Neonates with Congenital Osteochondrodysplasia(ocd/ocd) : II Functional Abnormalities in Central Nervous System and Respiratory Muscle
- Study on Cause of Death in Rat Neonates with Congenital Osteochondrodysplasia (ocd/ocd) : I. Morphological Abnormalities in Lung and Respiratory Tract
- External and Skeletal Observations on a New Lethal Mutant of the Rat : Congenital Osteochondrodysplasia with Systemic Subcutaneous Edema
- Control of trophoblast cell differentiation ; Lessons from the genetics of early pregnancy loss and trophoblalst neoplasia
- MINC: Multistage Interconnection Network with Cache Control Mechanism
- Expression of Basic Fibroblast Growth Factor-Like Substance in Immature Rat Testis: Immunocytochemical Localization and Detection of mRNA
- Basic Fibroblast Growth Factor-Like Substance Associates with DNA in Germ Cell Nuclei
- Prolonged Effect of 5-Fluorouracil and Its Derivatives on Apoptosis Induction and Mitotic Inhibition in the Intestinal Epithelium of Male BDF1 Mice.
- Preparation and Characterization of Highly $C$-Axis-Oriented Ba2NaNb5O15 Thin Film on La0.05Sr0.95TiO3 Substrate
- Structural and Ferroelectric Properties of Ba2NaNb5O15 Thin Films on La-Doped SrTiO3 Substrates
- Changes in 20.ALPHA.-Hydroxysteroid Dehydrogenase mRNA in the Ovary of Immature Rats Bearing a Single Generation of Corpora Lutea.
- Changes in Two Kinds of 20.ALPHA.-hydroxysteroid Dehydrogenase During the Luteal Phase in the Rat.
- Temporal and Spatial Distribution of Alkaline Phosphatase Activity in Male Hypogonadic Rat (hgn/hgn) Testis during Postnatal Development.