BUTANOL EXTRACTS FROM MYELIN FRAGMENTS: TRYPTAMINE BINDING TO LIPID FRACTIONS

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概要

• 論文の詳細を見る

• To investigate the nature of the tryptamine binding components that originated from myelin butanol extracts (i.e., myelin proteolipids), the lipid mixtures obtained from these extracts were further fractionated by silicic acid column chromatography, and binding assays of <SUP>14</SUP>C-tryptamine to those fractions were carried out by Sephadex LH<SUB>20</SUB> column chromatography. Among several lipid fractions, only the F-C fraction retained the tryptamine binding properties of the original myelin butanol extracts, i.e., binding capacity, chromatographic profile and interaction with indoleamine analogues and other neurotransmitters. Since the quantitative TLC analysis indicated that this fraction contained a considerable amount of phosphatidylserine (PS) and phosphatidylinositol (PI), recombination experiments with these two acidic lipids were planned. The recombination system with PI did not show a tryptamine binding capacity, while the PS fraction possessed a tryptamine binding capacity similar to that of the myelin butanol extracts. However, displacement studies revealed that the recombinant fraction with PS alone did not display the complete regeneration of the specificity which had been observed in the myelin extracts. All these observations infer that the tryptamine binding components from myelin proteolipids are lipid in nature, and its binding entity is mainly PS. However, some specifically organized constitution with PS and other lipid molecule(s) may be necessary to regenerate the original tryptamine binding properties.

• 社団法人 日本薬理学会の論文

著者

• KARASAWA AKIRA

  Group of Neuropharmacology, Faculty of Pharmaceutical Sciences, Josai University

• ISHITANI RYOICHI

  Group of Neuropharmacology, Faculty of Pharmaceutical Sciences, Josai University

• KIM Su-Ja

  Group of Biochemical Pharmacology, Josai University

• KARASAWA Akira

  Group of Biochemical Pharmacology, Josai University

• ISHITANI Ryoichi

  Group of Biochemical Pharmacology, Josai University

関連論文

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• TETRAHYDROAMINOACRIDINE SHOWS A TROPHIC EFFECT ON DIFFERENTIATING CEREBELLAR GRANULE CELLS
• BUTANOL EXTRACTS FROM MYELIN FRAGMENTS: TRYPTAMINE BINDING TO “QUAKING” MICE-MYELIN BUTANOL EXTRACTS
• BUTANOL EXTRACTS FROM MYELIN FRAGMENTS: TRYPTAMINE BINDING TO LIPID FRACTIONS
• Butanol extracts from myelin fragements: some characteristics of tryptamine binding.
• Effect of Phospholipase A2 on Temperature-Induced High-Affinity (3H)Tryptamine Binding Sites in Rat Brain.
• Effects of protein-modifying reagents on brain tryptamine binding sites: Possible involvement of a thiol group in temperature-induced high-affinity (3H)tryptamine binding sites.
• (3H)5-hydroxytryptamine binding to reconstituted fraction with sulphatides, phosphatidylserine and phosphatidylinositol.

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