Effect of Phospholipase A2 on Temperature-Induced High-Affinity (3H)Tryptamine Binding Sites in Rat Brain.
スポンサーリンク
概要
- 論文の詳細を見る
To investigate a link between membrane phospholipids and tryptamine binding molecules, we examined the effects of phospholipases A<SUB>2</SUB> and D on the temperature-sensitive high-affinity [<SUP>3</SUP>H]tryptamine binding sites in rat brain. When the phospholipase A<SUB>2</SUB>-treated membranes were exposed to 1% bovine serum albumin (BSA) before assaying for [<SUP>3</SUP>H]tryptamine binding, a complete dose-dependent inhibition curve was observed. At a concentration of 0.03 U, the action of phospholipase A<SUB>2</SUB> resulted in the splitting of phosphatidylserine (PS), choline phosphatides (PC) and ethanolamine phosphatides (PE) by about 32, 34 and 65%, respectively, and reduced [<SUP>3</SUP>H]ligand binding by about 32%. On the contrary, in the case of phospholipase D (500 U), PS and PC decreased by about 8% and 33% and PE by about 29% with no significant alteration in the binding capacity. Moreover, Scatchard analysis of the [<SUP>3</SUP>H]tryptamine binding showed that phospholipase A<SUB>2</SUB> drastically increased only the K<SUB>D</SUB> value of the high affinity sites, and this was accompanied by a decrement of the B<SUB>max</SUB> values of both the high and low affinity binding sites. From these results, it is inferred that certain lipids (PS) may be a modulator for the function of the temperature-induced high-affinity [<SUP>3</SUP>H]tryptamine binding molecules.
- 公益社団法人 日本薬理学会の論文
著者
-
ISHITANI Ryoichi
Group of Biochemical Pharmacology, Josai University
-
Ishitani Ryoichi
Group of Neurophartnaeology, Josai University
-
Saito Michiko
Group of Neurophartnaeology, Josai University
-
Serikyaku Shigefumi
Group of Neurophartnaeology, Josai University
-
SAITO Michiko
Group of Neuropharmacology, Josai University
-
SERIKYAKU Shigefumi
Group of Neuropharmacology, Josai University
関連論文
- [^3H] TRYPTAMINE BINDING TO RECONSTITUTED FRACTION OF ACIDIC LIPIDS
- TETRAHYDROAMINOACRIDINE SHOWS A TROPHIC EFFECT ON DIFFERENTIATING CEREBELLAR GRANULE CELLS
- BUTANOL EXTRACTS FROM MYELIN FRAGMENTS: TRYPTAMINE BINDING TO LIPID FRACTIONS
- Butanol extracts from myelin fragements: some characteristics of tryptamine binding.
- Effect of Phospholipase A2 on Temperature-Induced High-Affinity (3H)Tryptamine Binding Sites in Rat Brain.
- Effects of protein-modifying reagents on brain tryptamine binding sites: Possible involvement of a thiol group in temperature-induced high-affinity (3H)tryptamine binding sites.
- (3H)5-hydroxytryptamine binding to reconstituted fraction with sulphatides, phosphatidylserine and phosphatidylinositol.