(3H)5-hydroxytryptamine binding to reconstituted fraction with sulphatides, phosphatidylserine and phosphatidylinositol.
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概要
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As a first step toward the examination of the involvement of sulphatides, phosphatidylserine and phosphatidylinositol in 5-HT receptor mechanisms, we performed [<SUP>3</SUP>H]5-HT binding experiments on the various reconstituted fractions with these acidic lipids. A binding assay of [<SUP>3</SUP>H]5-HT to these fractions was carried out by Sephadex LH<SUB>20</SUB> column chromatography. Among various reconstituted fractions, only the reconstitution system with the three acidic lipids exhibited a saturable [<SUP>3</SUP>H]5-HT binding capacity, whereas no binding was seen with [<SUP>3</SUP>H]-spiperone. When the binding of [<SUP>3</SUP>H]5-HT to this fraction was plotted as a function of the ligand concentration, a multiple binding mode with three classes of binding components (or sites) was observed. Furthermore, the double reciprocal plot indicated that this reconstitution system had three apparent K<SUB>D</SUB> values of 4.7, 15 and 59 nM. The displacement studies with various compounds indicated that only a few 5-HT agonists (5-methoxytryptamine and tryptamine) and neurotransmitters (DA and ACh) inhibited the[<SUP>3</SUP>H]5-HT binding to this fraction, but 5-HT antagonists, LSD analogues and neuroleptics had no effect. Moreover, GTP, GDP and Gpp(NH)p clearly inhibited the[<SUP>3</SUP>H]5-HT binding in spite of their weak potencies, while GMP did not have any effect.
- 公益社団法人 日本薬理学会の論文
著者
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KARASAWA Akira
Group of Biochemical Pharmacology, Josai University
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ISHITANI Ryoichi
Group of Biochemical Pharmacology, Josai University
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ISHITANI Ryoichi
Group of Neuropharmacology, Josai University
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NISHI Akira
Group of Neuropharmacology, Josai University
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KARASAWA Akira
Group of Neuropharmacology, Josai University
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YOSHIKAWA Shigeka
Group of Neuropharmacology, Josai University
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- (3H)5-hydroxytryptamine binding to reconstituted fraction with sulphatides, phosphatidylserine and phosphatidylinositol.