Butanol extracts from myelin fragements: some characteristics of tryptamine binding.

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概要

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• Myelin fragments from rat brain stems were treated with butanolwater mixtures and binding experiments of <SUP>14</SUP>C-tryptamine to these extracts (i.e. proteolipids) were performed by Sephadex LH<SUB>20</SUB> column chromatography. The elution peak of <SUP>14</SUP>C-tryptamine appeared in a boundary between 6:1 and 4:1 chloroform-methanol, together with 19.2 and 7.3% of the total recoveries of protein and lipid phosphorus, respectively. Various compounds were studied to examine their inhibitory effects on the tryptamine binding to these extracts. The results indicated that only tryptamine and 5-methoxytryptamine inhibited the tryptamine binding, but indole analogues and other neurotransmitters had no effect. The kinetic analysis revealed that the tryptamine binding components present in the myelin butanol extracts are composed of saturable and non-saturable components, and the saturable binding components had an apparent Kd of 1.14×10<SUP>-7</SUP> M. As a preliminary study, myelin butanol extracts were separated into a lipid and protein fractions with ice-cold ether treatment, and then the <SUP>14</SUP>C-tryptamine binding capacities of both fractions were examined. The results indicated that only the lipid fraction possessed a tryptamine binding capacity and a chromatographic profile similar to the original butanol extracts. Moreover, the heat-treated preparation of myelin extracts also retained the tryptamine binding capacity. All these observations suggest that the myelin butanol extracts have a specific binding capacity for tryptamine, and its binding components may be lipid in nature.

• 公益社団法人 日本薬理学会の論文

著者

• KAMEYAMA TSUTOMU

  Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Meijo University

• ISHITANI RYOICHI

  Group of Neuropharmacology, Faculty of Pharmaceutical Sciences, Josai University

• KIM Su-Ja

  Group of Biochemical Pharmacology, Josai University

• Kameyama Tsutomu

  Department Of Biological Chemistry Faculty Of Pharmaceutical Sciences Nagoya City University

• MIYAKAWA Akihisa

  Group of Biochemical Pharmacology, Josai University

• ISHITANI Ryoichi

  Group of Biochemical Pharmacology, Josai University

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