Occurrence of degradation products of chicken transferrin in murine circulating blood.
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概要
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To determine the true survival of an exogenous substance in the circulation, it is requisite to assess that of the intact molecules. We studied the survival of chicken transferrin (Tf) in murine circulating blood after i.v. injection. With the aid of polyacrylamide-gel isoelectric focusing, direct immunofixation and densitometry, degradation products of chicken Tfs were found, and the disappearance rate of intact molecules was rather higher than that obtained by the single radial immunodiffusion method. These results strongly suggest that the degradation products must be taken into consideration in studies on the clearance of proteinous substances.
- 公益社団法人 日本薬理学会の論文
著者
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Ozawa Eijiro
Division Of Cell Biology National Center For Nervous Mental And Muscular Disorders
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Saito Koji
Department Of Anesthesiology And Intensive Care Medicine Tohoku University Hospital
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Kimura Ichiro
Department Of Basic Human Sciences School Of Human Sciences Waseda University
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