TOXICITY OF LOCAL ANAESTHETICS ON MYOGENIC CELLS IN CULTURE
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概要
- 論文の詳細を見る
Toxicity of local anaesthetics on chick myogenic cells (mononucleated myoblasts and multinucleated myotubes) in culture was examined. Following treatment with the drugs, myogenic cells showed some morphological changes and finally detached from the culture dishes. In most cases, the toxic effect was estimated by the amount of cells detached quantitatively. The indices used to show the amount of cells were the deoxyribonucleic acid (DNA) and creatine kinase activity content of mono- and multinucleated cells remaining on the dishes, respectively. Dibucaine was more toxic than bupivacaine, mepivacaine, tetracaine and procaine, and was examined in detail. The toxicity was dependent on its concentration, pH and temperature of the reaction medium in both mono- and multinucleated cells, and paralleled the concentration of uncharged form of the drug, suggesting that this form in external medium was actually toxic.
- 公益社団法人日本薬学会の論文
著者
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OZAWA EIJIRO
Division of Cell Biology, National Center for Nervous, Mental and Muscular Disorders
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Hagiwara Yasuko
Division Of Cell Biology National Center For Nervous Mental And Muscular Disorders
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Ozawa E
Division Of Cell Biology National Center For Nervous Mental And Muscular Disorders
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Ozawa Eijiro
Division Of Cell Biology National Center For Nervous Mental And Muscular Disorders
関連論文
- REVERSIBLE SUPPRESSION OF GROWTH AND DIFFERENTIATION OF CULTURED CHICK MYOGENIC CELLS WITH VERY LOW CONCENTRATIONS OF DIBUCAINE
- TOXICITY OF LOCAL ANAESTHETICS ON MYOGENIC CELLS IN CULTURE
- DESTRUCTION AND GROWTH SUPPRESSION OF CULTURED MYOGENIC CELLS BY DIBUCAINE
- Fe3+ promotes in vitro growth of myoblasts and other cells from chick embryos.
- Basis for the assay of myogenic cell growth in vitro using creatine kinase activity as an index, with special reference to measurement of power ratio of transferrins in growth promotion.
- Avian and mammalian transferrins are required for chick and rat myogenic cell growth in vitro, respectively.
- Contraction of cultured chick myotube before acquisition of cell membrane excitability.
- Transferrin is an essential component of chick embryo extract for avian myogenic cell growth in vitro.
- Suppression of RNA synthesis following transferrin removal in chick myotubes.
- Occurrence of degradation products of chicken transferrin in murine circulating blood.
- Positive Immunostaining with Dystrophin Antibodies in mdx Skeletal Muscle.