上咽頭由来上皮性細胞への Epstein-Barr Virus Virus(EBV)感染成立 : オートラジオグラフィーの利用

概要

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The EBV genome has been shown to be present inside tumor cells of nasopharyngeal carcinoma. Accordingly, it was reasonable to assume that EBV infection may possibly occur in epithelial cells of the nasopharynx by some sort of biological mechanism. The authors carried out the experiments of EBV infection to nasopharyngeal epithelial cells. Firstly cell fusion was attempted between lymphoid cells (BJAB cells) which originated from a case of Burkitts lymphoma and freed from any of the EBV nucleic acid and adenoid-origin epithelial cells (227-Ad cells). Then, these fused cells were injected with EBV. It was found that EBV-associated nuclear antigen (EBNA) immunofluorescence could be detected only in the BJAB nuclei in the heterokaryons in the first experimental day. From the second day of post-infection, there was a gradual increase in the percentage of 2-27-Ad nuclei in the heterokaryons. However, there was no instance in which the EBNA was found only in a 2-27-Ad nucleus of a heterokaryon.
社団法人日本耳鼻咽喉科学会の論文