細胞培養による扁桃の抗原情報伝達系に関する研究

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It is important to make the immunological role of the tonsil clear, comparing the responses of tonsil with those of the spleen and lymphnodes which are known as peripheral lymphatic organs.These experiments were condutcd to learn whether the antigenic information in the tonsil induced the antibody formation of anothe lymphatic organs. The experimental procedures are as follows:1) Adult rabbits were sensitized intravenously and peritonsillarlly with SRBC in vivo, and tonsil, spleeen and lymphnode cells were cultured in vitro. Simultaneously, non-sensitized spleen cells were cultured with sensitized tonsil cells, with sensitized spleen cells and with sensitized lymphnode cells.2) 4 days after, the antibody forming cells (plaque forming cells) were meassured by Jernes hemolytic plaque technique.The following conclusions were drawn from this study:1) Live cells of 42% were harvested after 4-day culture.2) PFC number per 106 wore away to 1/2-1/10, in both single culture and mixed culture.3) On the whole, mixed culture was inclined to increase its number of PFC.4) In the group of one-time i. v. sensitization, only IgM-PFC of tonsil increased in number in mixed culture.5) In the group of repeated i. v. sensitization, every organ had the tendency of increase in number of PFC.6) In the group of peritonsillar sensitization, a small number of PFC was recognized in tonsil.Especially none of IgM-PFC was found in single culture, but mixed culture resulted is appearance of PFC.Discussions were done on the mechanism of the increase in number of PFC in mixed culture, and the ability of transfer of antigenic information of the tonsil.
社団法人日本耳鼻咽喉科学会の論文

細胞培養による扁桃の抗原情報伝達系に関する研究

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