Clinical Application of the Polymerase Chain Reaction for a Rapid Diagnosis of Mycobacterium tuberculosis Infection.
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概要
- 論文の詳細を見る
A gene amplification method of Mycobacterium tuberculosis DNA by the polymerase chain reaction (PCR) has been devised. A primer pair used in this study is 5'GTTGCCGTGGCGG TATCGG3' and 5'GCGACATTACGGGGCAGGTGG3', which brackets a 152-base region encoding the 65KD antigen, and a specific probe is 5'TTTGGGGTCATCTTTGGAGCG3'. The procedure could be completed within 2 days. The specificity and the sensitivity of the PCR for M. tuberculosis complex in identifying M. tuberculosis complex did not conflict with the conventional methods at all. Using this method, we could diagnose three cases of the disease, which had been very difficult to diagnose by the conventional methods, by detecting the DNA from the blood, liver biopsy specimen, lung aspirate, and pleural effusion.(Internal Medicine 31 : 1016-1022, 1992)
- 社団法人 日本内科学会の論文
著者
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Kimura Satoshi
The Department Of Diabetes And Metabolism Faculty Of Medicine Tokyo University
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OKA Shinichi
the Department of Infectious Diseases and Applied Immunology, the Institute of Medical Science, University of Tokyo
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KIKUCHI Yoshimi
the Department of Infectious Diseases and Applied Immunology, the Institute of Medical Science, University of Tokyo
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MITAMURA Keiji
the Department of Infectious Diseases and Applied Immunology, the Institute of Medical Science, University of Tokyo
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SHIMADA Kaoru
the Department of Infectious Diseases and Applied Immunology, the Institute of Medical Science, University of Tokyo
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MITAMURA Keiji
the Department of Infectious Diseases, Institute of Medical Science, University of Tokyo
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OKA Shinichi
the Department of Infectious Diseases, Institute of Medical Science, University of Tokyo
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