Continuous Detection of Superoxide In Situ during Ischemia and Reperfusion in the Rabbit Heart.

概要

論文の詳細を見る
In order to clarify the time course of superoxide generation in situ duringischemia and reperfusion in the rabbit heart, we used a method of enhanced chemiluminescence (CL) with 2-methyl-6-[p-methoxyphenyl]-3, 7-dihydroimidazo[1, 2-α]pyrazin-3-one (MCLA) as a specific probe for detecting superoxide radicals. The surface of the rabbit heart was exposed to a photomultiplier tube in a light-proof box. We introduced a reversible snare occluder into the box to continuously observc thelight emission.An ischemia-reperfusion group (I/R, n=7)was subjected to 30mins of coronary occlusion, followed by 90mins of reperfusion. We performed the same procedure (except for coronary occlusion) in the sham-operated group (n=4). Another group of rabbits(n=4) subjected to I/R received superaxide dismutase (SOD: 20mg/kg, iv)during reperfusion to observe the CL response. In the I/R-group, the increase in CL began at 13±2 (mean±SEM) mins and peaked at 52±12mins of reperfusion. GL in the I/R-group gradually increased from 818±350counts/10 secs in the preischemic period to 1077±401counts/10 secs during reperfusion (p<0.01). In contrast, there was no increase in CL in the sham-operated group. The administration of SOD briefly attenuated CL by 24.1±6.8% for a period of 24.3±6.8mins. The superoxide generation in situ in the ischemic rabbit heart appears to increase gradually and persists for a period following reperfusion.
International Heart Journal刊行会の論文