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Influences of nitrogen source and the culture concentration of nitrogen, phosphorus and potassium on the growth of tea plant, the constitution of amino acids of leaves and mineral elements of plant organs were investigated by sand culture method carried out for two years. The concentration of nitrogen (NH4-N, NO3-N), phosphorous and potassium were set to be 0, 25, 50 and 100 ppm, respectively. The results obtained were summarized as follows. 1) The tea plant cultured with 25 and 50 ppm of each mineral nutrient showed better growth, that with 100 ppm of each element exhibited excess damage, and that without each nutrient showed the respective deficiency symptom, especially enhanced one in the case of nitrogen. The tea plant supplied with NH4-N as nitrogen source showed better growth than that with NO3-N. The leaves cultured by NO3-N showed chlorosis in any concentration. 2) As for the constitution of amino acids, the leaf supplied with no nitrogen contained small amount of each amino acid. In the case of NH4-N, the higher the nitrogen concentration, the more the contents of theanine, arginine, serine, threonine and so on. On the contrary, in the case of NO3-N, the contents of theanine, arginine, serine, etc. slightly increased according to the amount of nitrogen in the first year of the experiment, but remarkably reduced in the second year. As for amino acids of leaf supplied with no phosphorus, a large amount of arginine accu-mulated in it. The higher the phosphrous concentration, the less the contents of arginine, serine and so on. The higher the potassium concentration, the more the serine content, but the other amino acids tended to decrease. 3) The mineral elements of each organ increased according to each mineral nutrient. The amount of total nitrogen was more abundant in each organ of the plant supplied with NH4-N, and this increase rate was higher in the case of NH4-N than that of NO3-N. The manganese content was higher in the aerial part than the subterranean one of the plant supplied with NH4-N, whereas it was higher in the subterranean part than the aerial one in the case of NO3-N, and the content increased with the increase of the cocentration of supplied nitrogen. The aluminium content of each organ was higher in the tea plant supplied with NH4-N. The iron content was also higher in each organ of the tea plant supplied with NH4-N, especially in the root. Antagonism of potassium and zinc against NH4-N, that of magnesium and manganese against potassium, and that of zinc against phosphorus were recognized, these trends being remarkable in the root.
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