反芻胃内消化に及ぼす牧草抽出区分の影響 : III. 第一胃内微生物の窒素代謝に対するラジノクローバー抽出液の作用
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Extract of dried Ladino clover powder which was prepared with the medium of dil. HCl and aceton (20:1) at pH 1-2 was infused into the rumen through the fistula after the neutralization with NaOH and the effects on the nitrogen metabolism and the gas formation exerted by the rumen bacteria were examined.First, this extract was given to two male and female goats which had been fed on the hay and concentrate through the fistula daily before feeding in the morning for one week. Then the next week the rumen contents were taken out before feeding and infusion of the extract in the morning, and they were filtrated through double gauze and resulting filtrate were centrifuged to remove large debris and protozoa.Then, casein, amino acids mixture and urea were added to this rumen liquor and were incubated at 39°C for 1-3 hours. And the proteolytic activity of rumen bacteria was examined by the estimation of the amount of protein which was consumed during incubation. Then the deamination of amino acid and the urease activity of rumen bacteria were examined by the method of estimation of ammonia.Two (W/V) percent of soluble starch was added to the rumen liquor which was put into fermentation tubes and the volume of the gas produced was estimated after the incubation at 39°C for three hours.As the results it was ascertained that there was not difference in the value of proteolysis, deamination and urease activity between the periods of before and after the administration of the extract. But the volumes of the gas produced were always greater at the time of infusion of the extract.The authors, therefore, concluded that the factors which were contained in the grass extract did not influence the activity of nitrogen metabolism but influenced the carbohydrate metabolism of which gas production was related.
- 社団法人 日本畜産学会の論文
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