球状蛋白質の熱的性質

概要

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Recent studies on thermal properties of globular proteins are reviewed with emphasis on conformational transitions.<BR>In many cases, thermal denaturation of globular proteins can be interpreted well by the two-state approximation whose validity has been tested calorimetrically. Significant deviations from the two-state approximation were observed in some cases, which shows the existence of stable intermediate states.<BR>Existence of a prodenaturational stage has been suggested by gradual increase of heat capacity prior to the denaturational stage.<BR>Thermal denaturation of the protein was also observed in a crystalline state. The melting temperature of crystalline lysozyme changes reversively from aobut 70 to 200°C depending upon the water content of the crystal. Merit of studies in a crystalline state is discussed.
日本生物物理学会の論文