Asymmetric Synthesis of (S)-α-Methylbenzylamine by Recombinant Escherichia coli Co-Expressing Omega-Transaminase and Acetolactate Synthase

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概要

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• To produce (S)-α-methylbenzylamine (MBA) from acetophenone, recombinant Escherichia coli co-expressing ω-transaminase and acetolactate synthase was used as a whole-cell biocatalyst. The solvent-bridge reaction system increased the yield of the whole-cell reaction by 2.5-fold, and the inhibitory (S)-α-MBA produced in the ω-transaminase reaction solution (pH 8.0) moved into the extraction solution (pH 3.0) via an organic solvent.

• 社団法人 日本農芸化学会の論文

著者

• Kim Byung-Gee

  School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Institut

• Yun Hyungdon

  School of Biotechnology, Yeungnam University

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