Kinetic Resolution of 3-Fluoroalanine Using a Fusion Protein of D-Amino Acid Oxidase with Vitroscilla Hemoglobin
スポンサーリンク
概要
- 論文の詳細を見る
In this study, a fusion protein (VHb-DAAO) of D-amino acid oxidase (DAAO) with Vitreoscilla hemoglobin (VHb) was functionally expressed in Escherichia coli and purified. The kcat value VHb-DAAO (47.1 s−1) towards rac-3-flouroalanine was about 2-fold higher than that of DAAO (21.9 s−1). rac-3-Flouroalanine (500 mM) was kinetically resolved into (R)-3-fluoroalanine with high enatiomeric excess (>99%) by VHb-DAAO with about 52% conversion.
- 社団法人 日本農芸化学会の論文
- 2011-04-23
著者
-
Yun Hyungdon
School of Biotechnology, Yeungnam University
-
Khang Yong-ho
School Of Biotechnology Yeungnam University
-
SEO Young-Man
School of Biotechnology, Yeungnam University
-
Seo Young-man
School Of Biotechnology Yeungnam University
-
Yun Hyungdon
School Of Biotechnology Yeungnam Univ.
-
Yun Hyungdon
School Of Biotechnology Yeungnam University
関連論文
- Asymmetric Synthesis of (S)-α-Methylbenzylamine by Recombinant Escherichia coli Co-Expressing Omega-Transaminase and Acetolactate Synthase
- Kinetic Resolution of 3-Fluoroalanine Using a Fusion Protein of D-Amino Acid Oxidase with Vitroscilla Hemoglobin
- Asymmetric Synthesis of (S)-α-Methylbenzylamine by Recombinant Escherichia coli Co-Expressing Omega-Transaminase and Acetolactate Synthase