Comparison of the ω-Transaminases from Different Microorganisms and Application to Production of Chiral Amines(Microbiology & Fermentation Technology)

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概要

• 論文の詳細を見る

• Microorganisms that are capable of (S)-enantioselective transamination of chiral amines were isolated from soil samples by selective enrichment using (S)-α-methyl-benzylamine ((S)-α-MBA) as a sole nitrogen source. Among them, Klebsiella pneumoniae JS2F, Bacillus thuringiensis JS64, and Vibrio fluvialis JS17 showed good ω-transaminase (ω-TA) activities and the properties of the ω-TAs were investigated. The induction level of the enzyme was strongly dependent on the nitrogen source for the strains, except for V. fluvialis JS17. All the ω-TAs showed high enantioselectivity (E>50) toward (S)-α-MBA and broad amino donor specificities for arylic and aliphatic chiral amines. Besides pyruvate, aldehydes such as propionaldehyde and butyraldehyde showed good amino acceptor reactivities. All the ω-TAs showed substrate inhibition by (S)-α-MBA above 200 mм. Moreover, substrate inhibition by pyruvate above 10 mм was observed for ω-TA from V. fluvialis JS17. In the case of product inhibition, acetophenone showed much greater inhibitions than L-alanine for all ω-TAs. Comparison of the enzyme properties indicates that ω-transaminase from V. fluvialis JS17 is the best one for both kinetic resolution and asymmetric synthesis to produce enantiomerically pure chiral amines. Kinetic resolution of sec-butylamine (20 mм) was done under reduced pressure (150 Torr) to selectively remove an inhibitory product (2-butanone) using the enzyme from V. fluvialis JS17. Enantiomeric excess of (R)-sec-butylamine reached 94.7% after 12 h of reaction.

• 社団法人日本農芸化学会の論文
• 2001-08-23

著者

• Kim Byung-Gee

  School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Institut

• Kim Byung-gee

  School Of Chemical Engineering And Institute For Molecular Biology And Genetics Seoul National Unive

• Kim Byung-gee

  School Of Chemical & Biological Engineering Seoul National University

• Shin Jong-shik

  School Of Chemical Engineering And Institute For Molecular Biology And Genetics Seoul National Unive

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