Contractile Characteristics of Rat Mesenteric Artery after Organ Culture
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概要
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Organ culture of blood vessels is a useful technique to analyze long-term effects of drugs. Various growth factors are responsible for structural and functional changes during vascular remodeling. We investigated 1) basic contractile characteristics in organ-cultured rat mesenteric arteries (MA) in serum-free condition and 2) long-term effects of fetal bovine serum (FBS). Rat isolated MA with [E (+)] or without [E ()] endothelium were divided into 1) freshly isolated (fresh), 2) cultured for 3 days without FBS (control) or 3) with 10% FBS (FBS). In E (+) control, maximal contraction by noradrenaline (NA), endothelin (ET)-1 and 5-hydroxytriptamine (5-HT) was similar to that in fresh. In E () control, maximal contraction by NA decreased whereas that by ET-1 and 5-HT didnt change from fresh. In E (+) FBS, maximal contraction by NA, ET-1, and 5-HT increased from control. In E () FBS, maximal contraction by NA and ET-1 decreased whereas that by 5-HT increased. In E (+) or E () control and FBS, sensitivity to NA and ET-1 increased from fresh. In E (+) and E () control, sensitivity to 5-HT decreased from fresh, and that in FBS further decreased from control. Three-day organ-cultured rat MA in serum-free condition preserved enough contraction to enable analysis for long-term effects of drug. In FBS, maximal contractions by NA and ET-1 increased in E (+) and decreased in E () from control, while those by 5-HT increased in both E (+) and E ().
- 社団法人 日本獣医学会の論文
著者
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Yamawaki Hideyuki
Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Kitasato University, Japan
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Okada Muneyoshi
Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Kitasato University, Japan
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Hara Yukio
Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Kitasato University, Japan
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Yamawaki Hideyuki
東京大学 農生命科研究
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Yamawaki Hideyuki
Department Of Veterinary Pharmacology School Of Veterinary Medicine Kitasato University
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MORITA Tomoka
Laboratory of Crop Science, Program of Agronomy, Department of Bioresource and Bioenvironment, Schoo
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Okada Muneyoshi
Department Of Veterinary Pharmacology Graduate School Of Agriculture And Life Sciences The Universit
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Okada Muneyoshi
Department Of Veterinary Pharmacology School Of Veterinary Medicine And Animal Sciences Kitasato Uni
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Morita Tomoka
Laboratory Of Crop Science Program Of Agronomy Department Of Bioresource And Bioenvironment School O
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Okada Muneyoshi
Laboratory Of Veterinary Pharmacology School Of Veterinary Medicine Kitasato University
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Hara Yukio
Laboratory Of Veterinary Pharmacology School Of Veterinary Medicine Kitasato University
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YAMAWAKI Hideyuki
Laboratory of Veterinary Pharmacology, Kitasato University, Towada, Aomori 034–8628, Japan
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MORITA Tomoka
Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Kitasato University
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