Influences of Organic Solvents on CYPMPO-Electron Spin Resonance Spectra in In Vitro Radical Generating Systems

概要

論文の詳細を見る
Estimation of radical scavenging capacity of lipophilic antioxidants by electron spin resonance (ESR) in vitro is a challenging issue due to their poor solubility in aqueous radical generating and measuring systems. Water-miscible organic solvents are used for this propose. A novel radical trapping agent, 5-(2,2-dimethyl-1,3-propoxy cyclophosphoryl) -5-methyl-1-pyrroine N-oxide (CYPMPO) which has practical advantages over well-known trapping agents was synthesized. However, no available data for the influence of solvents in the CYPMPO-used ESR system is presented. Influences of six water-miscible organic solvents, acetonitrile (AcN), acetone, dimethyl sulfoxide (DMSO), ethanol, polyethylene glycol (PEG), and dimethoxyethane (DME), on ESR responses to Fenton Fe2+/H2O2 OH• and hypoxanthine/xanthine oxidase superoxide generation systems in vitro were studied. Reduction of the ESR signal to CYPMPO-OH• adducts by 44.14 ± 5.95% and 16.83 ± 2.50% from control was observed in the presence of AcN and acetone, respectively, at a final concentration of 5% (v/v). AcN less than 1% had minimal effects. DMSO, ethanol, PEG, and DME at 5% (v/v) strongly inhibited the ESR signals and/or caused derangement in the signal patterns. The six water-miscible solvents at 5% (v/v) had no influence on the ESR spectra of CYPMPO-superoxide adducts. From these results, AcN, less than 1% (v/v), is a useful water-miscible organic solvent for assessing radical scavenging capacities of lipophilic compounds in CYPMPO-Fenton Fe2+/H2O2 OH• reaction system in ESR assy. Any solvents used in the present study are available in hypoxanthine/xanthine oxidase superoxide generation system.

Influences of Organic Solvents on CYPMPO-Electron Spin Resonance Spectra in In Vitro Radical Generating Systems

スポンサーリンク

概要

著者

関連論文

スポンサーリンク