Home-cage activity, Open-field, Passive avoidanceテストで得られた正常コントロールデータに基づくENU優性行動変異体のスクリーニング
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概要
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To establish the cutoff values for screening ENU-induced behavioral mutations, normal variations in mouse behavioral data were examined in home-cage activity (HA), open-field (OF), and passive-avoidance (PA) tests. We defined the normal range as one that included more than 95% of the normal control values. The cutoffs were defined to identify outliers yielding values that deviated from the normal by less than 5% for C57BL/6J, DBA/2J, DBF1, and N2 (DXDB) progenies. Cutoff values for G1-phenodeviant (DBF1) identification were defined based on values over ± 3.0 SD from the mean of DBF1 for all parameters assessed in the HA and OF tests. For the PA test, the cutoff values were defined based on whether the mice met the learning criterion during the 2nd (at a shock intensity of 0.3 mA) or the 3rd (at a shock intensity of 0.15 mA) retention test. For several parameters, the lower outliers were undetectable as the calculated cutoffs were negative values. Based on the cutoff criteria, we identified 275 behavioral phenodeviants among 2,646 G1 progeny. Of these, 64 were crossed with wild-type DBA/2J individuals, and the phenotype transmission was examined in the G2 progeny using the cutoffs defined for N2 mice. In the G2 mice, we identified 15 novel dominant mutants exhibiting behavioral abnormalities, including hyperactivity in the HA or OF tests, hypoactivity in the OF test, and PA deficits. Genetic and detailed behavioral analysis of these ENU-induced mutants will provide novel insights into the molecular mechanisms underlying behavior.
著者
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SHIROISHI Toshihiko
Mammalian Genetics Laboratory, Genetic Strains Research Center, National Institute of Genetics
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和田 由美子
筑波大学
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和田 由美子
理化学研究所ゲノム科学総合研究センターゲノム機能情報研究グループ
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Kobayashi Kimio
Technol. And Dev. Team For Mouse Phenotype Analysis Japan Mouse Clinic Riken Bioresource Center
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Noda Tetsuo
Team For Advanced Dev. And Evaluation Of Human Disease Models Riken Bioresource Center
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Shiroishi Toshihiko
Mammailan Genetics Laboratory National Institute Of Genetics
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和田 由美子
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource
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古瀬 民生
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource
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山田 郁子
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource
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MASUYA Hiroshi
Technology and Development Unit for Knowledge Base of Mouse Phenotype, RIKEN BioResource Center
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KUSHIDA Tomoko
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource
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SHIBUKAWA Yoko
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource
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NAKAI Yuji
Mutagenesis and Genomics Team, RIKEN BioResource Center
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KANEDA Hideki
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource
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GONDO Yoichi
Mutagenesis and Genomics Team, RIKEN BioResource Center
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WAKANA Shigeharu
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource
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Gondo Yoichi
Mutagenesis And Genomics Team Riken Bioresource Center
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Masuya Hiroshi
Technol. And Dev. Unit For Knowledge Base Of Mouse Phenotype Riken Bioresource Center
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WADA Yumiko
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource
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FURUSE Tamio
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource
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YAMADA Ikuko
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource
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Wakana Shigeharu
Technol. And Dev. Team For Mouse Phenotype Analysis Japan Mouse Clinic Riken Bioresource Center
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SHIBUKAWA Yoko
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource Center
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NODA Tetsuo
Team for Advanced Development and Evaluation of Human Disease Models, RIKEN BioResource Center
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WAKANA Shigeharu
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource Center
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KUSHIDA Tomoko
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource Center
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YAMADA Ikuko
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource Center
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FURUSE Tamio
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource Center
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KOBAYASHI Kimio
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource Center
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KANEDA Hideki
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource Center
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