C57BL/6J凍結精子を用いた個体復元に効果的な体外受精法
スポンサーリンク
概要
- 論文の詳細を見る
A large number of genetically modified mouse strains have been produced in recent years. Sperm cryopreservation is the most effective means of preserving these valuable strains, most of which have a C57BL/6 genetic background. However, the fertilization efficiency of sperm from several cryopreserved strains, including C57BL/6, is quite low. While new and improved methods of cryopreservation have been developed, the majority of sperm stocks have already been cryopreserved using traditional methods, such as storage in 18% raffinose and 3% skim milk (R18S3). Therefore, new thawing methods for these frozen stocks are needed. We have developed a new thawing method that involves selective collection of motile sperm and a preincubation medium that enhances capacitation. Motile sperm are selected simply by collecting a sample from the center of a dish, and capacitation is induced by the addition of methyl-beta-cyclodextrin, D-penicillamine, sodium citrate, and hypotaurine to modified Tyrodes solution. The fertilization rate of sperm prepared using this method was increased significantly compared to that of sperm thawed using the traditional method (63.9 vs 16.5%, P<0.01). These results demonstrate that this new in vitro fertilization method is an effective means of reviving C57BL/6 sperm cryopreserved in R18S3.
著者
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Kobayashi Kimio
Technol. And Dev. Team For Mouse Phenotype Analysis Japan Mouse Clinic Riken Bioresource Center
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Ogura Atsuo
Bioresource Center Riken
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KANEDA Hideki
Technology and Development Team for Mouse Phenotype Analysis, Japan Mouse Clinic, RIKEN BioResource
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Wakana Shigeharu
Technol. And Dev. Team For Mouse Phenotype Analysis Japan Mouse Clinic Riken Bioresource Center
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Mochida Keiji
Bioresource Center Riken
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TAGUMA Kyuichi
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center
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NAKAMURA Chika
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center
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OZAKI Ai
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center
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SUZUKI Chigusa
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center
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HACHISU Akiko
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center
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田熊 究一
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center
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中村 千佳
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center
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尾崎 藍
Technology and Development Team for Mouse Phenotype Analysis, RIKEN BioResource Center
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OGURA Atsuo
Bioresource Engineering Division, RIKEN BioResource Center
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