遺伝子改変マウス21系統の体外受精系におけるピエゾマイクロマニピュレーターを用いた透明帯部分切開術(ZIP)の効果
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概要
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Zona incision using a piezo-micromanipulator (ZIP) has been demonstrated to be effective for in vitro fertilization (IVF) using cryopreserved C57BL/6 spermatozoa. In this study, ZIP oocytes inseminated with frozen-thawed genetically modified C57BL/6J or FVB mice spermatozoa (21 lines) showed fertilization rates of 22-75% and live fetus rates of 8-49%. In 6 of the lines, the fertilization rates for oocytes compared with ZIP (42-75%) were significantly higher than that of nontreated oocytes (0-50%). Using only 90 oocytes for IVF with ZIP, 5 breeding pairs were produced from cryopreserved genetically modified mice spermatozoa. Our results indicate that application of the ZIP technique is effective for IVF using cryopreserved genetically modified mouse spermatozoa.
著者
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川瀬 洋介
Chugai Research Institute for Medical Science
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立部 貴典
Chugai Research Institute for Medical Science
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羽仁 俊夫
Chugai Research Institute for Medical Science
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TATEISHI Hiromi
Chugai Research Institute for Medical Science
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JISHAGE Kou-ichi
Chugai Research Institute for Medical Science
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SUZUKI Hiroshi
Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro Univ
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Kawase Yosuke
Chugai Research Institute For Medical Science
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Kawase Yosuke
Chugai Pharmaceutical Co. Ltd.
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TACHIBE Takanori
Chugai Research Institute for Medical Science
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HANI Toshio
Chugai Research Institute for Medical Science
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Suzuki Hiroshi
Research Group For Neutron Scattering From Functional Materials Neutron Science Research Center Japa
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Tachibe Takanori
Chugai Res. Inst. For Medical Sci.
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Jishage Kou-ichi
Chugai Res. Inst. For Medical Sci.
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Suzuki Hiroshi
Research Center, Taisho Pharmaceutical Co., Ltd.,
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