Generation of Adeno-Associated Virus Vector Enabling Functional Expression of Oxytocin Receptor and Fluorescence Marker Genes Using the Human eIF4G Internal Ribosome Entry Site Element
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概要
- 論文の詳細を見る
We developed the AAV-Oxtr-IRES-Venus vector to rescue the oxytocin receptor (Oxtr) gene functionally at restricted regions in the brains of Oxtr knockout mice. First we chose human eIF4G gene-derived IRES to co-express Venus, a fluorescent marker gene, with Oxtr. With selected human eIF4G IRES, we constructed the AAV-Oxtr-IRES-Venus vector, and it caused expression of the Venus gene in the brain when 1 μl of viral solution (9.4×107 vg) was injected into the medial amygdaloid nucleus. In primary neuronal cells transduced with this viral vector and followed by oxytocin administration, functional expression of OXTR was detected by Ca2+ imaging assay.
- 社団法人 日本農芸化学会の論文
著者
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Ozawa Keiya
Division of Genetic Therapeutics, Jichi Medical School
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Mizukami Hiroaki
Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical School
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KASAHARA Yoshiyuki
Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University
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Sato Keisuke
Laboratory Of Molecular Biology Department Of Molecular And Cell Biology Graduate School Of Agricult
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DATE Shiori
Laboratory of Molecular Biology, Department of Molecular and Cell Biology, Graduate School of Agricu
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AOYAGI Yumi
Laboratory of Molecular Biology, Department of Molecular and Cell Biology, Graduate School of Agricu
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NAWA Akihiko
Department of Obstetrics and Gynecology, Graduate School of Medicine, Nagoya University
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HIDEMA Shizu
Laboratory of Molecular Biology, Department of Molecular and Cell Biology, Graduate School of Agricu
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NISHIMORI Katsuhiko
Laboratory of Molecular Biology, Department of Molecular and Cell Biology, Graduate School of Agricu
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