Experimental Photodynamic Therapy for Liver Cancer Cell-Implanted Nude Mice by an Indole-3-acetic Acid and Intense Pulsed Light Combination
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概要
- 論文の詳細を見る
Recently, indole-3-acetic acid (IAA) has been introduced as a new cancer therapeutic agent through oxidative decarboxylation by horseradish peroxidase (HRP). The purpose of this study was to determine the therapeutic feasibility of IAA/light combination against liver cancer. SK-HEP-1 cells were irradiated with UVB or visible light (518 nm) in the presence of IAA. Cell viability was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Then, IAA was injected in SK-HEP-1 liver cancer cell-implanted nude mice, and the tumor area was irradiated with intense pulsed light (IPL). Then, tissue was taken for terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) assay and immunohistochemical staining for 8-hydroxy-deoxyguanosine (8-OHdG), p53, caspase-3, and proliferating cell nuclear antigen (PCNA). In vitro experiments demonstrated that IAA alone was not cytotoxic, but activated IAA by HRP or light caused cell death. In vivo experiments showed that IAA/IPL treatment caused regression of tumor cells in SK-HEP-1-implanted nude mice. The TUNEL assay showed that IAA/IPL induced cancer cell apoptosis, and this was confirmed by increases in 8-OHdG, p53, and caspase-3 in IAA/IPL-treated mice. In contrast, IPL alone did not induce apoptosis, indicating that the apoptotic effect resulted from activated IAA by light. In summary, we showed that IAA/light induced tumor regression in SK-HEP-1-implanted nude mice. These results suggest the potential use of IAA/light combination in liver cancer.
- 日本薬学会の論文
著者
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Kim Dong-seok
韓国
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Park K
Department Of Dermatology Seoul National University College Of Medicine And Department Of Dermatolog
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Kim So-young
韓国
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Kim So
Department Of Dermatology Seoul National University College Of Medicine
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Kim Dong
Department Of Dermatology Seoul National University College Of Medicine:research Division For Human
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KIM Dong-Seok
Department of Dermatology, Seoul National University College of Medicine
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PARK Kyoung-Chan
Department of Dermatology, Seoul National University College of Medicine
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KIM So-Young
Department of Dermatology, Seoul National University College of Medicine
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Kim So-young
Department Of Biochemistry And Molecular Biology Laboratory Of Cellular Signaling Cancer Research In
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Park Kyoung-chan
Department Of Dermatology Seoul National University College Of Medicine And Seoul National Universit
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Park Kyoung
Department Of Dermatology Seoul National University College Of Medicine Clinical Research Institute
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Park Kyoung-chan
Departments Of Dermatology Seoul National University College Of Medicine
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Kim Dong
Department Of Dermatology And Artificial Organ Laboratory Clinical Research Institute Seoul National
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Park Kyoung-chan
Department Of Dermatology Seoul National University Bundang Hospital
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Kim Dong-seok
Department Of Dermatology Seoul National University Bundang Hospital
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Kim So-young
Department Of Biochemistry And Molecular Biology Cancer Research Center Seoul National University Co
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Park Kyoung
Department Of Dermatology Seoul National University College Of Medicine Seoul National University Ho
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Kim Dong-seok
Department Of Biochemistry Chung-ang University College Of Medicine
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Kim Dong-Seok
Department of Biochemistry, College of Medicine, Chung-Ang University
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Kim So-Young
Department of Biochemistry, College of Medicine, Chung-Ang University
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