Effect of Resveratrol on Helicobacter pylori-Induced Interleukin-8 Secretion, Reactive Oxygen Species Generation and Morphological Changes in Human Gastric Epithelial Cells

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概要

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• Inflammatory cytokine interleukin-8 (IL-8) and reactive oxygen species (ROS) overexpressed in the gastric mucosa when exposed to Helicobacter pylori, defined as a class I carcinogen. Moreover, infection with H. pylori leads to morphological changes in co-cultured cells known as hummingbird phenomenon along with increased motility. Resveratrol, a highly abundant polyphenol in red grapes, has shown anti-inflammatory, anti-cancer, cardioprotective and neuroprotective activities. However, the effect of resveratrol in H. pylori-infected cells has not been investigated. The present study was, therefore, aimed to evaluate the effect of resveratrol on the induction of IL-8, ROS and hummingbird morphology in H. pylori-infected gastric epithelial cells. The non-toxic concentration of resveratrol for both H. pylori and epithelial cells was determined by brucella broth dilution method and DNA fragmentation assay. The non-toxic resveratrol (≤100 μM) treatment did not demonstrate any inhibitory effect against H. pylori adhesion to gastric epithelial cells. However, preincubation of the cells with 75 and 100 μM of resveratrol significantly (p<0.05 and p<0.01 respectively) inhibited the secretion of IL-8 from H. pylori-infected cells. In addition, resveratrol pretreatment at 1—100 μM suppressed H. pylori-induced ROS generation in a concentration dependent manner. Moreover, H. pylori-initiated morphological changes were markedly blocked by resveratrol. Hence, resveratrol can be considered as a potential candidate against various H. pylori related gastric pathogenic processes.

• 日本薬学会の論文

著者

• Yamamoto Takeshi

  Division of Cardiology, Department of Medicine and Clinical Science, Yamaguchi University Graduate S

• Kondo Takashi

  Department Of Radiological Sciences Graduate School Of Medicine And Pharmaceutical Sciences Universi

• Zaidi Syed

  Department Of Medicine University Health Network & University Of Toronto

• Sugiyama Toshiro

  Department Of Gastroenterology And Hematology Graduate School Of Medicine And Pharmaceutical Science

• Sugiyama Toshiro

  Department Of Gastroenterology And Hematology Faculty Of Medicine University Of Toyama

• Ahmed Kanwal

  Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, Univer

• Usmanghani Khan

  Department of Basic Clinical Sciences, Faculty of Eastern Medicine, Hamdard University

• Kadowaki Makoto

  Division of Gastrointestinal Pathophysiology, Institute of Natural Medicine, University of Toyama

• Usmanghani Khan

  Department Of Basic Clinical Sciences Faculty Of Eastern Medicine Hamdard University

• Kondo Takashi

  Department Of Diagnostic Radiology Tohoku University School Of Medicine

• Ahmed Kanwal

  Department Of Radiological Sciences Graduate School Of Medicine And Pharmaceutical Sciences Universi

• Kadowaki Makoto

  Division Of Gastrointestinal Pathophysiology Department Of Bioscience Institute Of Natural Medicine

• Yamamoto Takeshi

  Division Of Cardiology Department Of Medicine Nippon Medical School

• Kondo Takashi

  Department Of Applied Physics Faculty Of Engineering The University Of Tokyo

• Yamamoto Takeshi

  Division Of Cardiology Department Of Internal Medicine Nippon Medical School

• Kondo Takashi

  Department Of Agricultural Chemistry The University Of Tokyo

• Sugiyama Toshiro

  Department Of Child Psychiatry University Of Tokyo Hospital

• Zaidi Syed

  Department of Gastroenterology and Hematology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama

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