Immature Rat Uterotrophic Assay of Diethylstilbestrol, Ethynyl Estradiol and Atrazine
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概要
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The purpose of this study was to decide the appropriate administration period in uterotrophic assay and to evaluate the usefulness of uterotrophic assay using two typical estrogen agonists and one antagonist, when chemicals were given orally. Diethylstilbestrol (DES), ethynyl estradiol (EE), or Atrazine (ATZ) was administered to immature Crj: CD (SD) IGS rats orally at doses of 0.01-1 μg/kg/day, 0.06-6 μg/kg/day, or 0.5-50 mg/kg/day from postnatal day (PND) 21 for 3 or 7 days, respectively. Additional groups of rats given ATZ orally at the above doses were also treated with 17β-estradiol (E2) subcutaneously at a dose of 0.3 μg/rat/day. A vehicle control group given only olive oil was established in each study, and a group given only E2 was included in the study of ATZ also. No abnormal clinical signs were detected in any of the groups. There were no significant differences in body weight between the vehicle control and each of the groups given DES, EE or ATZ, or the group given only E2 and each of the groups given ATZ plus E2. Premature vaginal opening was observed from 6 days in all rats given 6 μg/kg EE or ATZ plus E2. Uterine fluid was also present in all rats given 6 μg/kg EE or ATZ plus E2 for 3 or 7 days. Uterine weight was significantly higher in the rats given 1 μg/kg DES for 3 days and 6 μg/kg EE for 3 or 7 days, but no increases were detected in any of the rats given DES for 7 days. Although uterotrophic activity was not detected in any of the rats given only ATZ, co-treatment with 50 mg/kg ATZ and E2 for 3 days reduced the E2-induced increases in uterine weight. The present results demonstrated that the appropriate period of oral administration for detecting the estrogenic properties of DES and EE or anti-estrogenic properties of ATZ using the immature rat uterotrophic assay was 3 days and ATZ exerted weak anti-estrogenic effects on the uterus.
- 日本毒性病理学会の論文
- 1999-12-01
著者
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MAEKAWA Akihiko
Sasaki Institute
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NODA Shuji
Chemicals Evaluation and Research Institute
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MUROI Takako
Chemicals Evaluation and Research Institute
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YAMASAKI Kanji
Chemicals Evaluation and Research Institute
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Yamasaki Ken-ichi
Institute Of Laboratory Animals Faculty Of Medicine Kyoto University
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Maekawa Akihiko
Sasaki Research Laboratory Department Of Pathology Sasaki Institute
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SAWAKI Masakuni
Chemicals Assessment Center, Chemicals Evaluation and Research Institute
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Noda Shuji
Chemicals Evaluation And Research Institute Chemicals Assessment Center
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Yamasaki Kanji
Chemicals Assessment Center Chemicals Evaluation And Research Institute
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Sawaki Masakuni
Chemicals Assessment Center Chemicals Evaluation And Research Institute
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Takatuki Mineo
Chemicals Evaluation And Research Institute
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Yamasaki Kanji
Hita Laboratory Chemicals Evaluation And Research Institute
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Takatsuki Mineo
Chemicals Evaluation And Research Institute
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Yamasaki Ken-ichi
Inst. Of Lab. Animals Graduate School Of Medicine Kyoto Univ.
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Noda Shuji
Chemicals Assessment Center, Chemicals Evaluation and Research Institute, 3-822, Ishii, Hita, Oita 877-0061, Japan
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Muroi Takako
Chemicals Assessment Center, Chemicals Evaluation and Research Institute, 3-822, Ishii, Hita, Oita 877-0061, Japan
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