A selectable marker using cytochrome P450 monooxygenases for Arabidopsis transformation
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概要
- 論文の詳細を見る
We have developed a selection system for transformation of Arabidopsis thaliana via Agrobacterium tumefaciens. This selection system uses human cytochrome P450 monooxygenases that metabolize herbicides. Herbicide-tolerant seedlings transformed with CYP1A1, CYP2B6, CYP2C9, or CYP2C19 were selected with the herbicides acetochlor, amiprophos-methyl, chlorpropham, chlorsulfuron, norflurazon, and pendimethalin. The herbicide-tolerant plants transformed with CYP1A1, CYP2B6, and CYP2C19 expressed the corresponding P450 cDNAs. Inheritance and segregation of the P450 genes were analyzed in T2 progeny of herbicide-tolerant T1 seedlings. Metabolism of [14C]norflurazon by transgenic T2 seedlings expressing CYP1A1 produced non-toxic N-demethylated norflurazon. This result suggests that the P450 species expressed in transgenic Arabidopsis plants coordinately functioned as selectable markers because of active metabolism of the herbicides.
- 日本植物細胞分子生物学会の論文
著者
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Inui H
Research Center For Environmental Genomics Kobe University
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OHKAWA Hideo
Research Center for Environmental Genomics, Kobe University
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今石 浩正
Research Center For Environmental Genomics Kobe University
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OHKAWA Yasunobu
National Institute of Agrobiological Resources
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INUI Hideyuki
Research Center for Environmental Genomics, Kobe University
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Yamada Ryu
Graduate School of Science and Technology, Kobe University
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Yamada Tetsuya
National Institute of Agrobiological Sciences, Ministry of Agriculture, Forestry and Fisheries
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