Absence of strand bias for deletion mutagenesis during chromosomal leading and lagging strand replication in Escherichia coli
スポンサーリンク
概要
- 論文の詳細を見る
Investigations were carried out to determine whether both DNA strands involved in Escherichia coli chromosomal DNA replication are replicated with similar accuracy. Experiments consisted of measuring the forward mutation rate from tonB+ to tonB– in pairs of polA deficient strains in which the chromosomal target gene tonB was oriented in the two possible directions relative to the origin of replication, oriC. Within these pairs, the tonB sequence would be subjected to leading strand replication in one orientation and to lagging strand replication in the other. The most common tonB mutations in the polA1 strain were deletions followed by frameshifts. Among the deletions, a strong hotspot site with a 13-base deletion in the polA1 strains accounted for 18 of the 33 deletions in the one orientation, and 31 of the 58 deletions in the other. The results suggested that the two strands were replicated with equal or similar accuracy for deletion formation.
- 日本遺伝学会の論文
- 2005-02-01
著者
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Nagata Yuki
Graduate School Of Life Sciences Tohoku University
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Nagata Yuki
Graduate School Of Dentistry (department Of Orthodontics) Osaka Dental University
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WATANABE Takashi
Graduate School of Biomedical Engineering, Tohoku University
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Imai Masaru
Graduate School Of Life Sciences Tohoku University
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Ihara Makoto
Atomic Bomb Disease Institute Nagasaki University Graduate School Of Biomedical Sciences
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Yamamoto Kazuo
Graduate School Of Life And Environmental Sciences Osaka Prefecture University
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Kawata Masakado
Graduate School Of Life Sciences Tohoku University
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Tago Yu-ichiro
Graduate School of Life Sciences, Tohoku University
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KAWAGUCHI Genta
Graduate School of Life Sciences, Tohoku University
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SAKURAI Shigehisa
Graduate School of Life Sciences, Tohoku University
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Tago Yu-ichiro
Graduate School Of Life Sciences Tohoku University
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Kawaguchi Genta
Graduate School Of Life Sciences Tohoku University
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Sakurai Shigehisa
Graduate School Of Life Sciences Tohoku University
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Imai Masaru
Graduate School Of Life Sciences Tohoku Univ.
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Watanabe Takashi
Graduate Department Of Mathematical & Life Sciences Hiroshima Univ.
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