Partial Purification and Some Properties of Spore Germination Promoter(s) for Dictyostelium discoideum Excreted by Klebsiella aerogenes(Microbiology & Fermentation Industry)

概要

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Spore germination promoter(s) for the cellular slime mold Dictyostelium discoideum was partially purified from the culture medium of Klebsiella aerogenes using Amberlite XAD-2 and molecular sieve gel column chromatography. The active components were found both outside and inside of a dialyzing bag and were separated into several fractions on a Sephadex G-75 gel column. The treatment of partially purified promoter(s) with NaIO_4 oxidation resulted in the complete loss of the activity. The promoter(s) was very unstable against heat treatment at pH 12. Several enzymes, such as proteinase K, RNase, DNase, lipase, and zymolyase could not inactivate the promoter(s). Interestingly, the spore germination depended on phosphate ion at 25-100 niM and proteose peptone or yeast extract in addition to the promoter(s).
社団法人日本農芸化学会の論文
1990-10-23