Effects of Saccharomyces cerevisiae mec1, tel1, and mre11 mutations on spontaneous and methylmethane sulfonate-induced genome instability
スポンサーリンク
概要
- 論文の詳細を見る
In eukaryotes, together with the Mre11/Rad50/Xrs2 (or Nbs1) complex, a family of related protein kinases (the ATM family) is involved in checkpoint activation in response to DNA double-strand breaks. In Saccharomyces cerevisiae, two members of this family, MEC1 and TEL1, have functionally redundant roles in DNA damage repair. Strains with mutations in their mec1 as well as mre11 genes are very sensitive to DNA damaging agents, show defective induction of damage-induced cell-cycle checkpoints, and defective damage-induced homologous recombination. However, the fact that both the mec1Δ and mre11Δ strains exhibit the spontaneous hyper-recombination phenotype is paradoxical in light of the homologous recombination defects in these strains. In this study, we constructed yeast mec1, tel1, and mre11 null mutations and characterized their genome stability properties. Spontaneous and methylmethane sulfonate (MMS)-induced point mutations, base-substitutions, and frameshifts occurred to an almost equal extent in the wild-type, mec1Δ, tel1Δ, and mre11Δ strains. Thus, Mec1, Tel1, and Mre11 do not play roles in the point mutation response. We then found that the mec1Δ, mre11Δ, and mec1Δ tel1Δ strains demonstrated increased rates of spontaneous loss of heterozygosity (LOH), which includes crossover, gene conversion, and chromosome loss, compared with the wild-type strain. In the tel1Δ strain, the rate of spontaneous LOH was as low as that in the wild-type strain. Finally, no induction of LOH by MMS was observed in the mec1Δ, mre11Δ, or mec1Δ tel1Δ strain; however, it was detected in the wild-type and tel1Δ strains upon exposure to MMS. The elevated level of spontaneous LOH but not MMS-induced LOH in the mec1Δ, mre11Δ, and mec1Δ tel1Δ strains suggests the presence of high levels of spontaneous recombinogenic DNA damage, which differs from the damage induced by MMS treatment, in these strains.
著者
-
Suetomi Kazuhiro
Graduate School of Life Sciences. Tohoku University
-
Mochizuki Mai
Graduate School of Life Sciences. Tohoku University
-
Suzuki Shiori
Graduate School of Life Sciences. Tohoku University
-
Yamamoto Hiroaki
Graduate School of Life Sciences. Tohoku University
-
Yamamoto Kazuo
Graduate School of Life Sciences. Tohoku University
-
Yamamoto Kazuo
Graduate School Of Life And Environmental Sciences Osaka Prefecture University
関連論文
- Effects of Saccharomyces cerevisiae mec1, tel1, and mre11 mutations on spontaneous and methylmethane sulfonate-induced genome instability
- Biochemical and biological properties of DNA photolyases derived from utraviolet-sensitive rice cultivars
- Hydroquinone, a Benzene Metabolite, Induces Hog1-dependent Stress Response Signaling and Causes Aneuploidy in Saccharomyces cerevisiae
- Temperature-Sensitive Photoreactivation of Cyclobutane Thymine Dimer in Soybean
- CPD Photolyase Gene from Spinacia oleracea : Repair of UV-Damaged DNA and Expression in Plant Organs
- Formation of Mg-Ni Hydrogen Absorption Alloy Film by Molten Salts Electrolysis (特集 New Trends in Molten Salt Chemistry and Electrochemistry)
- Generation of 'OH and oxidative DNA lesions in aerobically grown E. coli lacking both SOD and Fur
- Multiple origins and high genetic diversity of cultivated radish inferred from polymorphism in chloroplast simple sequence repeats
- Saccharomyces cerevisiae RAD27 complements its Escherichia coli homolog in damage repair but not mutation avoidance
- Induction of mitosis delay, apoptosis and aneuploidy in human cells by phenyl hydroquinone, an Ames test-negative carcinogen
- The Study of Mutagenecity of Thymine Glycol in Escherichia coli Mutant for Thymine Glycol Glycosylases
- Genetic diversity of cultivated and wild radish and phylogenetic relationships among Raphanus and Brassica species revealed by the analysis of trnK/matK sequence
- Spontaneous Mutagenesis in Escherichia coli and Saccharomyces cerevisiae
- Absence of strand bias for deletion mutagenesis during chromosomal leading and lagging strand replication in Escherichia coli
- Effects of the 5'- and 3'-Flanking bases on the Formation of G→T Transversion by 8-oxoG.
- 1-Nitropyrene Efficiently Induces Mitotic Recombination in Saccharomyces cerevisiae
- 1-Nitropyrene Efficiently Induces Mitotic Recombination in Saccharomyces cerevisiae
- Electrodeposition of Zn-Mg Alloy from Trimethyl Propylammonium Bis(trifluoromethylsulfonyl)imide Ionic Liquid
- Error-free RAD52 pathway and error-prone REV3 pathway determines spontaneous mutagenesis in Saccharomyces cerevisiae
- Activity of Zn Ionic Species and Electrodeposition of Zn in EMIB-ZnBr_2-Ethylene Glycol Bath
- Frameshift Mutations Produced by 9-Aminoacridine in Wild-Type, uvrA and recA Strains of Escherichia coli : Specificity Within a Hotspot
- Effects of Saccharomyces cerevisiae mec1, tel1, and mre11 mutations on spontaneous and methylmethane sulfonate-induced genome instability