イネ染色体における高頻度反復配列の構成〔英文〕

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概要

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• Digestion of the total genomic DNA from rice Oryza sativa L. cv. C5924 with EcoRI generated an intense band of a DNA fragment of about 0.36 kb long. The DNA fragment cloned into pUC19 was used to hybridize with the total rice genomic DNA partially digested with EcoRI. A ladder of bands of DNA fragments with multiplied length of 0.36 kb was observed, demonstrating that this sequence occurs in tandem in the genome. The copy number of the sequence estimated by dot blot hybridization analysis was 2000-3000 copies per haploid genome from callus or seedling of C5924. This sequence was present in other O. sativa cultivars, such as Sasanishiki in 700-900 copies, Koshihikari in 3400-4300, and Nipponbare in 4600-6000 copies. Another rice species, O. glaberrima, also had this sequence in 540-680 copies, but four lines of foxtail millet had none. The DNA fragments containing the repeated sequences in Nipponbare were then cloned into λ EMBL3, and sequences of nine units consecutively repeated and an AT-rich sequence connected with them in a phage clone could be determined. Each repeating unit showed sequence divergency mostly by substitution of bases in a range from 3% to 7%, when compared with a 355-bp consensus sequence. Analyses of the substituted bases indicate that these are due to spontaneous mutations which occurred at random, after reiteration of a unit sequence by unequal crossing over events. Gene conversion within the repeated sequences might have further diversified their sequences.

• 日本遺伝学会の論文

著者

• OHTSUBO Hisako

  Institute of Molecular and Cellular Biosciences, the University of Tokyo

• OHTSUBO Eiichi

  Institute of Molecular and Cellular Biosciences, the University of Tokyo

• UMEDA Masaaki

  Institute of Molecular and Cellular Biosciences,University of Tokyo

• Umeda Masaaki

  Institute Of Applied Microbiology University Of Tokyo

• Ohtsubo Hisako

  Institute Of Applied Microbiology University Of Tokyo

• Ohtsubo Eiichi

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

• 大坪 久子

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• OHTSUBO Eiichi

  Institute of Applied Microbiology, University of Tokyo

• 梅田 正明

  Institute of Applied Microbiology, University of Tokyo

• 大坪 栄一

  Institute of Applied Microbiology, University of Tokyo

関連論文

• Phylogenetic analysis of Oryza rufipogon strains and their relations to Oryza sativa strains by insertion polymorphism of rice SINEs
• Identification and characterization of Australian wild rice strains of Oryza meridionalis and Oryza rufipogon by SINE insertion polymorphism
• Two new SINE elements, p-SINE2 and p-SINE3, from rice
• [Plant BioTechniques Series (7)] A Rapid and Easy-handling Procedure for Isolation of DNA from Rice, Arabidopsis and Tobacco
• Expressed Sequence Tags in Developing Anthers of Rice (Oryza sativa L.)
• Molecular Characterization of Metallothionein Genes in Plants
• New SINE families from rice, OsSN, with poly(A) at the 3' ends
• Rice Retroposon p-SINE1 and Origin of Cultivated Rice
• Isolation and Expression Analysis of Petunia CURLY LEAF-Like Genes
• Evolutionary relationships among rice species with AA genome based on SINE insertion analysis
• Identification and structural analysis of SINE elements in the Arabidopsis thaliana genome
• Identification and characterization of petunia genes homologous to Arabidopsis CURLY LEAF gene
• Tnr8, a foldback transposable element from rice
• Molecular mechanism of the petunia class B gene function
• Identification and phylogenetic analysis of gypsy-type retrotransposons in the plant kingdom
• RIRE2, a novel gypsy-type retrotransposon from rice
• Analysis of Petunia hybrida genes homologous to the Arabidopsis CURLY LEAF gene
• INTERACTIONS BETWEEN PETUNIA FLORAL HOMEOTIC GENES BLIND AND GREEN PETAL
• RIRE1, a retrotransposon from wild rice Oryza australiensis
• Identification and characterization of two tandem repeat sequences(TrsB and TrsC)and a retrotransposon(RIRE1)as genome-general sequences in rice
• イネ染色体における高頻度反復配列の構成〔英文〕
• 可動性遺伝因子のイントロンへの挿入によるイネWaxy遺伝子の多様化〔英文〕
• イネ染色体に存在する転移性因子の同定と,その挿入領域の多型性に基づくイネの系統関係の解析〔英文〕
• New transposable elements identified as insertions in rice transposon Tnr1
• Isolation and characterization of a rice cDNA encoding B1-type cyclin-dependent kinase
• イネ染色体に存在する散在型反復配列p-SINE1ファミリ-の解析〔英文〕
• Tn3とγδのキメラトランスポゼ-スを用いたトランスポゼ-スの逆向き繰り返し配列(IR)に対する結合特異性決定部位の解析〔英文〕
• Cyclin-Dependent Protein Kinases and Their Regulators in Plants

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