RIRE2, a novel gypsy-type retrotransposon from rice
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概要
- 論文の詳細を見る
The 441-bp DNA segment in a PCR-amplified fragment from Oryza sativa cv. IR36 was found to have a sequence with features characteristic of LTRs of retroelements, which was named RIRE2 (Rice retroelement #2) and further analyzed. Cloning and sequencing analyses of the DNA segments connected to LTR-like sequence showed that RIRE2 has a long internal region almost 10 kb long that is flanked by LTR-like sequences. This internal region carries a primer binding site (PBS) and polypurine tract (PPT) which are necessary for cDNA synthesis of retroelements. The PBS sequence is complementary to the 3 end region of tRNAArg. The internal region has an rt gene homologous to that of gypsy-type retrotransposons, evidence that RIRE2 is indeed a retrotransposon related to gypsy from Drosophila. RIRE2 has an extra sequence more than 4 kb long in the region downstream of gag-pol. Phylogenetic analysis of the putative amino-acid sequences of the rt gene as well as the int gene showed that RIRE2 is related to a group of gypsy-type retrotransposons of a large size that include Grande1-4 of teosinte, Tat4-1 and Athila1-1 of Arabidopsis thaliana, and Cyclops-2 of pea, but distantly related to any other group of gypsy-type retrotransposons, including RIRE3 and RIRE8 of rice. RIRE2 and Grande1-4 had the highest homology in the gag-pol region, but the nucleotide sequences of the LTR regions differed. Both elements had significant homology in the middle area of the extra regions downstream of gag-pol, in which they had an open reading frame encoding a protein with no known function on the opposite strand from that coding for gag-pol.
- 日本遺伝学会の論文
著者
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OHTSUBO Hisako
Institute of Molecular and Cellular Biosciences, the University of Tokyo
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OHTSUBO Eiichi
Institute of Molecular and Cellular Biosciences, the University of Tokyo
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Ohtsubo E
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
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Ohtsubo H
Univ. Tokyo Tokyo Jpn
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Ohtsubo Hisako
Institute Of Applied Microbiology University Of Tokyo
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Ohtsubo Eiichi
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
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KUMEKAWA Norikazu
Institute of Molecular and Cellular Biosciences, the University of Tokyo
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Kumekawa Norikazu
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
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OHTSUBO Eiichi
Institute of Applied Microbiology, University of Tokyo
関連論文
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