可動性遺伝因子のイントロンへの挿入によるイネWaxy遺伝子の多様化〔英文〕

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概要

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• The waxy (wx) gene of Oryza glaberrima was cloned, and its nucleotide sequence was determined. A waxy mutant of O. glaberrima showing a glutinous phenotype was found to contain a substitution mutation generating a termination codon in the coding region of the wx gene. The Wx sequence of O. glaberrima was different from that of Oryza sativa by substitutions and insertions/deletions, among which only a few substitutions occurred in several exons not to severely alter the amino acid sequence of the Wx protein. The most striking difference observed in introns was a 139-bp deletion (or insertion) in intron 10 of O. glaberrima (or O. sativa). In O. sativa, 125bp of the 139-bp sequence was flanked by direct repeats of a 14-bp sequence. A sequence homologous to the 125-bp sequence was found in the region preceding exon 2; this sequence was also flanked by direct repeats of another 14-bp sequence. This result and the observation that the 125-bp sequence was interspersed in rice genomes indicate that they are SINEs (short interspersed elements) in the plant system. We also identified a DNA sequence with long terminal inverted repeats in intron 13 of both O. glaberrima and O. sativa. This sequence was present in multiple copies in rice genomes, suggesting that it is a transposable element. These results obtained suggest that mobile DNA elements have diversified the rice Waxy gene by inserting into introns, each of which may originally have a length of about 100 bp.

• 日本遺伝学会の論文

著者

• OHTSUBO Hisako

  Institute of Molecular and Cellular Biosciences, the University of Tokyo

• OHTSUBO Eiichi

  Institute of Molecular and Cellular Biosciences, the University of Tokyo

• UMEDA Masaaki

  Institute of Molecular and Cellular Biosciences,University of Tokyo

• Umeda Masaaki

  Institute Of Applied Microbiology University Of Tokyo

• Ohtsubo Hisako

  Institute Of Applied Microbiology University Of Tokyo

• Ohtsubo Eiichi

  Institute Of Molecular And Cellular Biosciences The University Of Tokyo

関連論文

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• [Plant BioTechniques Series (7)] A Rapid and Easy-handling Procedure for Isolation of DNA from Rice, Arabidopsis and Tobacco
• Expressed Sequence Tags in Developing Anthers of Rice (Oryza sativa L.)
• Molecular Characterization of Metallothionein Genes in Plants
• New SINE families from rice, OsSN, with poly(A) at the 3' ends
• Rice Retroposon p-SINE1 and Origin of Cultivated Rice
• Isolation and Expression Analysis of Petunia CURLY LEAF-Like Genes
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• Identification and structural analysis of SINE elements in the Arabidopsis thaliana genome
• Identification and characterization of petunia genes homologous to Arabidopsis CURLY LEAF gene
• Tnr8, a foldback transposable element from rice
• Molecular mechanism of the petunia class B gene function
• Identification and phylogenetic analysis of gypsy-type retrotransposons in the plant kingdom
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• Analysis of Petunia hybrida genes homologous to the Arabidopsis CURLY LEAF gene
• INTERACTIONS BETWEEN PETUNIA FLORAL HOMEOTIC GENES BLIND AND GREEN PETAL
• RIRE1, a retrotransposon from wild rice Oryza australiensis
• Identification and characterization of two tandem repeat sequences(TrsB and TrsC)and a retrotransposon(RIRE1)as genome-general sequences in rice
• イネ染色体における高頻度反復配列の構成〔英文〕
• 可動性遺伝因子のイントロンへの挿入によるイネWaxy遺伝子の多様化〔英文〕
• イネ染色体に存在する転移性因子の同定と,その挿入領域の多型性に基づくイネの系統関係の解析〔英文〕
• New transposable elements identified as insertions in rice transposon Tnr1
• Isolation and characterization of a rice cDNA encoding B1-type cyclin-dependent kinase
• イネ染色体に存在する散在型反復配列p-SINE1ファミリ-の解析〔英文〕
• Tn3とγδのキメラトランスポゼ-スを用いたトランスポゼ-スの逆向き繰り返し配列(IR)に対する結合特異性決定部位の解析〔英文〕
• Cyclin-Dependent Protein Kinases and Their Regulators in Plants

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