New transposable elements identified as insertions in rice transposon Tnr1
スポンサーリンク
概要
- 論文の詳細を見る
Tnr1 (235 bp long) is a transposable element in rice. Polymerase chain reactions (PCRs) done with a primer(s) that hybridizes to terminal inverted repeat sequences (TIRs) of Tnr1 detected new Tnr1 members with one or two insertions in rice genomes. Six identified insertion sequences (Tnr4, Tnr5, Tnr11, Tnr12, Tnr13 and RIRE9) did not have extensive homology to known transposable elements, rather they had structural features characteristic of transposable elements. Tnr4 (1767 bp long) had imperfect 64-bp TIRs and appeared to generate duplication of a 9-bp sequence at the target site. However, the TIR sequences were not homologous to those of known transposable elements, indicative that Tnr4 is a new transposable element. Tnr5 (209 bp long) had imperfect 46-bp TIRs and appeared to generate duplication of sequence TTA like that of some elements of the Tourist family. Tnr11 (811 bp long) had 73-bp TIRs with significant homology to those of Tnr1 and Stowaway and appeared to generate duplication of sequence TA, indicative that Tnr11 is a transposable element of the Tnr1/Stowaway family. Tnr12 (2426 bp long) carried perfect 9-bp TIRs, which began with 5-CACTA- -3 from both ends and appeared to generate duplication of a 3-bp target sequence, indicative that Tnr12 is a transposable element of the En/Spm family. Tnr13 (347 bp long) had 31-bp TIRs and appeared to generate duplication of an 8-bp target sequence. Two sequences, one the transposon-like element Crackle, had partial homology in the Tnr13 ends. All five insertions appear to be defective elements derived from autonomous ones encoding the transposase gene. All had characteristic tandem repeat sequences which may be recognized by transposase. The sixth insertion sequence, named RIRE9 (3852 bp long), which begins with 5-TG- -3 and ends with 5- -CA-3, appeared to generate duplication of a 5-bp target sequence. These and other structural features indicate that this insertion is a solo LTR (long terminal repeat) of a retrotransposon. The transposable elements described above could be identified as insertions into Tnr1, which do not deleteriously affect the growth of rice cells.
- 日本遺伝学会の論文
著者
-
OHTSUBO Hisako
Institute of Molecular and Cellular Biosciences, the University of Tokyo
-
OHTSUBO Eiichi
Institute of Molecular and Cellular Biosciences, the University of Tokyo
-
Ohtsubo Hisako
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
-
Ohtsubo Hisako
Institute Of Applied Microbiology University Of Tokyo
-
Ohtsubo Eiichi
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
-
HAN Chang-Gyun
Institute of Molecular and Cellular Biosciences, The University of Tokyo
-
FRANK Mary
Institute of Molecular and Cellular Biosciences, The University of Tokyo
-
Frank Mary
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
-
Han Chang-gyun
Institute Of Molecular And Cellular Biosciences The University Of Tokyo
関連論文
- Phylogenetic analysis of Oryza rufipogon strains and their relations to Oryza sativa strains by insertion polymorphism of rice SINEs
- Identification and characterization of Australian wild rice strains of Oryza meridionalis and Oryza rufipogon by SINE insertion polymorphism
- Two new SINE elements, p-SINE2 and p-SINE3, from rice
- New SINE families from rice, OsSN, with poly(A) at the 3' ends
- Rice Retroposon p-SINE1 and Origin of Cultivated Rice
- Isolation and Expression Analysis of Petunia CURLY LEAF-Like Genes
- Evolutionary relationships among rice species with AA genome based on SINE insertion analysis
- Identification and structural analysis of SINE elements in the Arabidopsis thaliana genome
- Identification and characterization of petunia genes homologous to Arabidopsis CURLY LEAF gene
- Tnr8, a foldback transposable element from rice
- Molecular mechanism of the petunia class B gene function
- Identification and phylogenetic analysis of gypsy-type retrotransposons in the plant kingdom
- RIRE2, a novel gypsy-type retrotransposon from rice
- Analysis of Petunia hybrida genes homologous to the Arabidopsis CURLY LEAF gene
- INTERACTIONS BETWEEN PETUNIA FLORAL HOMEOTIC GENES BLIND AND GREEN PETAL
- RIRE1, a retrotransposon from wild rice Oryza australiensis
- Identification and characterization of two tandem repeat sequences(TrsB and TrsC)and a retrotransposon(RIRE1)as genome-general sequences in rice
- イネ染色体における高頻度反復配列の構成〔英文〕
- 可動性遺伝因子のイントロンへの挿入によるイネWaxy遺伝子の多様化〔英文〕
- イネ染色体に存在する転移性因子の同定と,その挿入領域の多型性に基づくイネの系統関係の解析〔英文〕
- New transposable elements identified as insertions in rice transposon Tnr1
- イネ染色体に存在する散在型反復配列p-SINE1ファミリ-の解析〔英文〕
- Tn3とγδのキメラトランスポゼ-スを用いたトランスポゼ-スの逆向き繰り返し配列(IR)に対する結合特異性決定部位の解析〔英文〕