ピレスロイド系殺虫剤にエストロゲン様活性があることをin vitro評価系を組み合わせて判定した

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概要

• 論文の詳細を見る

• Pyrethroid insecticides are among the most commonly used classes of insecticides worldwide, but their endocrine disrupting activities remain unclear. Therefore, in the present study, we examined the estrogenic activities of pyrethroid insecticides in E-screen and competition binding assays. In addition, we measured estrogen receptor (ER) protein and pS2 mRNA levels in human breast cancer cells (MCF-7 BUS) to clarify the mechanism of their estrogenicity. Seven pyrethroid insecticides (bioallethrine, cypermethrin, deltamethrin, fenvalerate, permethrin, sumithrin, and tetramethrin) were tested because of their worldwide usage. In addition, 17β-estradiol was tested as a positive control. As expected, 17β-estradiol significantly increased MCF-7 BUS cell proliferation at concentrations of 10-11 M and above. Of the pyrethroid insecticides tested, only sumithrin increased MCF-7 BUS cell proliferation in a dose-dependent manner; the maximum induction of cell proliferation was observed at a dose of 10-5 M. In the anti-estrogenic activity test, bioallethrin, fenvalerate, and permethrin significantly inhibited 17β-estradiol-induced MCF-7 BUS cell proliferation at 10-6 M, a concentration comparable to the effective dose (10-9 M) of ICI 182,780, a pure ER antagonist. However, none of the pyrethroid insecticides competitively inhibited the binding of [3H]estradiol to rat uterus ERs in competition binding assays. Both 17β-estradiol (10-10 M) and sumithrin (10-5 M) decreased the levels of cytosolic ERα and ERβ protein expression significantly as compared with the vehicle control. In addition, 17β-estradiol (10-10 M) increased pS2 mRNA expression markedly, and sumithrin significantly increased pS2 mRNA levels in a dose-dependent manner. The other six compounds tested in the present study did not affect ER protein levels or pS2 mRNA levels. These results suggest that certain pyrethroid insecticides may be considered to be estrogen-like chemicals that act through pathways other than direct ER binding, and may function as endocrine modulators in both wildlife and humans.

• 日本繁殖生物学会の論文

著者

• KIM Tae

  Endocrine Toxicology Team, National Institute of Toxicological Research, Korea Food and Drug Adminis

• KANG Il

  Endocrine Toxicology Team, National Institute of Toxicological Research, Korea Food and Drug Adminis

• MOON Hyun

  Endocrine Toxicology Team, National Institute of Toxicological Research, Korea Food and Drug Adminis

• Kim Seung

  National Institute Of Toxicological Research Korea Food And Drug Administration

• LEE Su

  Endocrine Toxicology Division, National Institute of Toxicological Research, Korea Food and Drug Adm

• Han Soon

  Endocrine Toxicology Team, National Institute of Toxicological Research, Korea Food and Drug Adminis

• MOON Aree

  College of Pharmacy, Duksung Women's University

• Kim In

  Endocrine Toxicology Division, National Institute of Toxicological Research, Korea Food and Drug Adm

• Shin Jae

  Endocrine Toxicology Division, National Institute of Toxicological Research, Korea Food and Drug Adm

• Kim Hyung

  Endocrine Toxicology Division, National Institute of Toxicological Research, Korea Food and Drug Adm

• CHOI Kwang

  Endocrine Toxicology Division, National Institute of Toxicological Research, Korea Food and Drug Adm

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• ピレスロイド系殺虫剤にエストロゲン様活性があることをin vitro評価系を組み合わせて判定した
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