Transformation of Escherichia coli Mediated by Natural Phospholipids
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概要
- 論文の詳細を見る
Transformation system for Escherichia coli based upon introduction of plasmid DNA by natural phospholipids has been developed. Transformants are easily obtained by treatment with natural phospholipids such as phosphatidylethanolamine, phosphatidylcoline, and phosphatidylserine, where the presence of MgCl2 or CaCl2 is essential. This method of transformation is applicable not only for small plasmid pHSG399 (2.3 Kb) but also for giant plasmid R6K (100 Kb).
- 社団法人 日本農芸化学会の論文
- 2005-01-23
著者
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Sato Yuko
Laboratory Of Physiology Tsukuba College Of Technology
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Kurusu Yasurou
Laboratory Of Molecular Microbiology College Of Agriculture Ibaraki University
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Yoshikawa Kenichi
Department Of Physics Graduate School Of Science Kyoto University
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Yoshikawa Kenichi
Department Of Physics Graduate School Of Science Kyoto University And Crest
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KUMAZAWA Noriyuki
Faculty of Engineering, Ibaraki University
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Kumazawa N
Faculty Of Engineering Ibaraki University
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Sato Yuko
Laboratory Of Molecular Microbiology College Of Agriculture Ibaraki University
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Yoshikawa Kenichi
Department Of Dermatology Yamagata University School Of Medicine
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Yoshikawa Kenichi
Depart. Biomed. Info., Doshisya Univ.
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