Entrapping Polymer Chain in Light Well under Good Solvent Condition(Cross-disciplinary Physics and Related Areas of Science and Technology)
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概要
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During the last decade, the laser trapping technique has been actively applied to elucidate the property of macromolecules such as deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and cytoskeleton fibres. Due to the inherent difficulty in the direct trapping of single molecules at approximately 300K, most of the current studies on laser trapping utilize the experimental technique to grasp microbeads that are attached to a single macromolecule, instead of using direct trapping. A few studies have demonstrated the applicability of laser trapping for highly packed compact DNA under a 'poor' solvent condition without any beads. In the present study, we achieve laser trapping on a single DNA molecule under a 'good' solvent condition without any microbeads. The time-dependent change of the conformation accompanied by ON/OFF laser irradiation was measured and analysed in terms of the entrapment and release of a coiled polymer around a micrometer-sized potential.
- 社団法人日本物理学会の論文
- 2005-07-15
著者
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Matsuzawa Yukiko
Department Of Ecological Engineering Toyohashi University Of Technology
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Ichikawa Masatoshi
Department Of Biological Science And Technology Tokyo University Of Science
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Yoshikawa Kenichi
Depart. Biomed. Info., Doshisya Univ.
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