Temperature Adaptation of Bacillus subtilis by Chromosomal groEL Replacement

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概要

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• We investigated a temperature adaptation of Bacillus subtilis 168 in which chromosomal groEL was replaced with a psychrophilic groEL. This strain can grow at 50 °C but not at 51 °C, a temperature at which wild-type B. subtilis can grow. Using in vivo random mutagenesis by the B. subtilis mutator strain (mutS, mutM, mutY), two thermo-adaptants were isolated from the groEL substituted strain at 52 °C. They contained novel amino acid alterations in their ATP binding motif (T93I) and the inter-monomer contact (R285H) region of GroEL. These results suggest that GroEL participates in bacterial temperature adaptation.

• 2006-10-23

著者

• ENDO Ayako

  Laboratory of Molecular Microbiology, College of Agriculture, Ibaraki University

• SASAKI Mayumi

  Laboratory of Molecular Microbiology, College of Agriculture, Ibaraki University

• MARUYAMA Akihiko

  National Institute of Advanced Industrial Science and Technology (AIST)

• KURUSU Yasurou

  Laboratory of Molecular Microbiology, College of Agriculture, Ibaraki University

• Endo Ayako

  Laboratory Of Molecular Microbiology College Of Agriculture Ibaraki University

• Sasaki Mayumi

  Laboratory Of Molecular Microbiology College Of Agriculture Ibaraki University

• Kurusu Yasurou

  Laboratory Of Molecular Microbiology College Of Agriculture Ibaraki University

• Maruyama Akihiko

  National Institute For Advanced Industrial Science And Technology (aist Ibrf) Tsukuba

関連論文

• Temperature Adaptation of Bacillus subtilis by Chromosomal groEL Replacement
• Identification of in Vivo Substrates of the Chaperonin GroEL from Bacillus subtilis
• "Abiotic core" of Kakkonda geothermal system revealed by down-hole fluid sampling-depth limit of biosphere of a magmatic-hydrothermal system(Coevolution of Earth and Life, International Symposium on Origins of Life and Astrobiology)
• A Nonconserved Carboxy-Terminal Segment of GroEL Contributes to Reaction Temperature
• Genetic Transformation System for a Psychrotrophic Deep-sea Bacterium : Isolation and Characterization of a Psychrotrophic Plasmid
• Identification of in Vivo Substrates of the Chaperonin GroEL from Bacillus subtilis
• Characterization of Two Compatible Small Plasmids from Sphingobium yanoikuyae
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• Purification and Characterization of N-Acetylglucosamine-6-phosphate Deacetylase from a Psychrotrophic Marine Bacterium, Alteromonas Species
• Genetic Analysis of an Incomplete bio Operon in a Biotin Auxotrophic Strain of Bacillus subtilis Natto OK2
• Transformation of Escherichia coli Mediated by Natural Phospholipids

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