Genetic Analysis of an Incomplete bio Operon in a Biotin Auxotrophic Strain of Bacillus subtilis Natto OK2
スポンサーリンク
概要
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We describe the genetic analysis of the bio operon of the biotin auxotrophic Bacillus subtilis natto OK2 strain. The OK2 strain would only cross-feed with the Escherichia coli bioB mutant and also grew well in medium containing dethiobiotin. Sequencing analysis revealed two significant genetic alterations in the bioW and bioF genes within the bio operon of the OK2 strain. Complementation analysis with B. subtilis 168 bio mutants demonstrated that only the bioB gene could complement, but other bio operon genes could not. A bio+ transformant, isolated from an OK2 strain, has biotin autotrophy.
- 社団法人 日本農芸化学会の論文
- 2004-03-23
著者
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SASAKI Mayumi
Laboratory of Molecular Microbiology, College of Agriculture, Ibaraki University
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KURUSU Yasurou
Laboratory of Molecular Microbiology, College of Agriculture, Ibaraki University
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Sasaki Mayumi
Laboratory Of Molecular Microbiology College Of Agriculture Ibaraki University
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Kurusu Yasurou
Laboratory Of Molecular Microbiology College Of Agriculture Ibaraki University
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Kawamura Fujio
Laboratory Of Molecular Genetics College Of Science Rikkyo (st. Paul's) University
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Kawamura Fujio
Laboratory Of Molecular Genetics And Research Information Center For Extremophiles College Of Scienc
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