Homogeneous Enzymatic Assay for L-Cysteine with βC-S Lyase
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概要
- 論文の詳細を見る
We have developed a new enzymatic assay for determining L-cysteine concentration. The method involves the use of βC-S lyase from Streptococcus anginosus, which catalyzes the α,β-elimination of L-cysteine to hydrogen sulfide, pyruvate, and ammonia. The production of pyruvate is measured by D-lactate dehydrogenase and NADH. The decrease in NADH was proportional to the L-cysteine concentration up to 1.0 mM. When serum samples were used, within-day and day-to-day coefficient variations were below 4%. This method is simple, and can easily and reliably be used for accurate determination of L-cysteine concentration in serum or other samples.
- 社団法人 日本農芸化学会の論文
- 2005-11-23
著者
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NAKANO Yoshio
Department of Environmental Chemical and Engineering, Interdisciplinary Graduate School of Science a
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Nishiya Yoshiaki
Department Of Biochemistry Toyobo Co. Ltd.
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Yoshida Yasuo
Department Of Applied Quantum Physics Kyushu University
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YOSHIMURA Mamiko
Department of Preventive Dentistry, Kyusyu University, Faculty of Dental Science
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FUKAMACHI Haruka
Department of Preventive Dentistry, Kyusyu University, Faculty of Dental Science
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Fukamachi Haruka
Department Of Preventive Dentistry Kyusyu University Faculty Of Dental Science
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Yoshimura Mamiko
Department Of Preventive Dentistry Kyusyu University Faculty Of Dental Science
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Yoshida Yasuo
Department Of Applied Quantum Physics Faculty Of Engineering Kyushu University
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Yoshida Yasuo
Department Of Preventive Dentistry Kyusyu University Faculty Of Dental Science
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Nakano Yoshio
Department Of Preventive Dentistry Kyusyu University Faculty Of Dental Science
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Nakano Yoshio
Department Of Chemical Engineering Faculty Of Engineering Shizuoka University
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