Effects of Serum Deprivation on Expression of Proteolytic-Related Genes in Chick Myotube Cultures
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概要
- 論文の詳細を見る
We previously reported that serum deprivation stimulates myofibrillar proteolysis in chick myotubes. In the present study, we examined the effect of serum deprivation on expression of the proteolytic-related genes (ubiquitin, proteasome, calpains, and cathepsin B) by real-time PCR of cDNA in chick myotubes. Myotubes were incubated with serum-free medium for 24 h. Ubiquitin and proteasome subunits (C1 and C2) and calpains (m-, μ-, and p94/calpain-3) but not cathepsin B mRNA expression were increased by serum deprivation. These results indicate that serum deprivation stimulates ubiquitin-proteasome and calpain proteolytic pathways, resulting in an increase in myofibrillar proteolysis in chick myotubes.
- 社団法人 日本農芸化学会の論文
- 2005-03-23
著者
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Abe Hiroyuki
National Inst. Of Livestock And Grassland Sci. Jpn
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YAMAZAKI Makoto
National Institute of Livestock and Grassland Science
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Namai Kazuo
Ibaraki Prefectural Livestock Res. Center Jpn
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NAKASHIMA Kazuki
Nutrient Function Laboratory, Department of Animal Physiology and Nutrition, National Institute of L
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YAMAZAKI Makoto
Nutrient Function Laboratory, Department of Animal Physiology and Nutrition, National Institute of L
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ABE Hiroyuki
Nutrient Function Laboratory, Department of Animal Physiology and Nutrition, National Institute of L
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NAKASHIMA Kazuki
Molecular Nutrition Research Team, National Institute of Livestock and Grassland Science
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Nakashima K
Molecular Nutrition Research Team National Institute Of Livestock And Grassland Science
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