Purification and Some Properties of Nuclease Inhibitor from Monascus purpureus

スポンサーリンク

概要

• 論文の詳細を見る

• An nuclease inhibitor was isolated from cells of Monascus purpureus after 2 days cultivation, and the inhibitor was released into the culture fluid after 6 days when cell autolysis occurred. The inhibitor was positive in ninhydrin and Anthrone tests. The inhibitor was purified by column chromatographies on Sephadex G-25, Biogel P-2 and DEAE-Sephadex A25, and was homogeneous on disc gel electrophoresis. The molecular weight of the inhibitor was estimated to be about 2500 by Sephadex G-75 gel filtration. The inhibitor reduced the activities of Monascus nuclease (nuclease MP) and nuclease PI, when RNA or heat-denatured DNA was used as substrate, but did not affect nucleotidase activity of either enzyme. Snake venom phosphodiesterase was also inhibited to some extent. The action mode of the inhibitor was noncompetitive inhibition. This inhibitor was named NMP inhibitor.

著者

• Kato Fumio

  Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Saga University

• Murata Akira

  Laboratory Of Applied Microbiology Department Of Agricultural Chemistry Saga University

• SARUNO Rinjiro

  Laboratory of Applied Microbiology, Faculty of Agriculture, Saga University

• SETOYAMA Tamotsu

  Laboratory of Applied Microbiology, Faculty of Agriculture, Saga University

• NAKASHIMA Chikako

  Laboratory of Applied Microbiology, Faculty of Agriculture, Saga University

関連論文

• Hydroxyl Radical as the Reactive Species in the Inactivation of Phages by Ascorbic Acid(Biological Chemistry)
• Mode of Action of Clostocin O Part 2. On the Synthesis of Nucleic Acid in Sensitive Bacteria
• Mode of Action of Clostocin O Part 1. On the Macromolecular Synthesis in Sensitive Bacteria
• Bound-Ligand Effect in the Complexation Kinetics of Hydroxozirconium(IV) and Hydroxohafnium(IV) Complexes
• Purification and Some Properties of Nuclease Inhibitor from Monascus purpureus
• A New Biological Activity in Vitro of Fat-soluble Vitamins and Related Substances: Phageinactivating Effect of Vitamins E and K, and Coenzyme Q
• Screening of Bacteriocins in AminoAcid or Nucleic Acid Producing Bacteria and Related Species
• Synthesis of Higher Homologs of Bifunctional L-Lysine Derivatives with Phage-inactivating Effect
• Synthesis of Bifunctional L-Lysine Derivatives with Phage-inactivating Effect

スポンサーリンク