Mechanisms of 5-Inosinic Acid Accumulation by Permeability Mutants of Brevibacterium ammoniagenes. I. Genetical Improvement of 5-IMP Productivity of a Permeability Mutant of B. ammoniagenes
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概要
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In order to establish industrial production of 5-inosmic acid (5-IMP), a permeability mutant, KY13171, of Brevibacterium ammoniagenes, which accumulated 7 to 8 grams of 5-IMP per liter and 4 to 6 grams of hypoxanthine (Hx) per liter (calculated as 5-IMP), was improved by a genetical procedure. Further improved mutants were selected stepwise through repeating mutational work. The finally selected mutant. KY13369, accumulated 20 to 27 grams of 5-IMP per liter, but not Hx. Increased productivity of 5-IMP and decreased productivity of Hx were not caused by the changes in 5-IMP degrading activity, because these activities were not significantly different among the mutants. These results appear to indicate that the increased accumulation of 5-IMP may be caused by the improvement in membrane permeability for 5-IMP. However, the changes in phospholipid and fatty acid compositions were not enough to explain the increased permeability.
- 社団法人 日本農芸化学会の論文
著者
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Teshiba Sadao
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Furuya Akira
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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