Effect of Growth Phase on Stability of Fumarase Activity of Brevibacterium flavum Cells Immobilized with κ-Carrageenan

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概要

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• Brevibacterium flavum cells obtained from different growth phases were immobilized with kcarrageenan and the stability of the fumarase activity was investigated. The stability of fumarase activity of the immobilized preparation of cells of the stationary growth phase was highest. The highest stability of the immobilized cells seemed to be correlated to the high stability of fumarase activity in free cells of the stationary phase. High rigidity of the cell wall and membrane of B. flavum cells of the stationary phase and firm binding of fumarase protein to the cell membranewere suggested from several lines of evidence obtained on treatment of the cells wi h lysozyme and detergents or sonication of the cells. Electronmicrographsshowedthat the cells of the stationary phase retained the original shape after repeated batch reactions. Solubilized fumarase prepared from cells of the stationary phase showedthe highest stability. Experiments using the partially purified enzymestrongly suggested the existence of fumarase-stabilizing components in the cells.

• 社団法人 日本農芸化学会の論文

著者

• Chibata Ichiro

  Department Of Biochemistry Research Laborabory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.

• Tosa Tetsuya

  Department Of Biochemistry Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.

• Takata Isao

  Department Of Biochemistry Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.

• TOSA Tetsuya

  Department of Biochemistry, Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd.

• TAKATA Isao

  Department of Biochemistry, Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd.

関連論文

• Preparation and Properties of Naringinase Immobilized by Ionic Binding to DEAE-Sephadex
• Improvement of Stability of Fumarase Activity of Brevibacterium flavum by Immobilization with κ-Carrageenen and Polyethyleneimine
• Stabilization of L-Aspartate β-Decarboxylase Activity of Pseudomonas dacunhae Immobilized with Carrageenan
• Isolation and Characterization of a Methanol-utilizing Bacterium Pseudomonas putida Strain 981
• Adsorption Specificity of Immobilized Tannin for Proteins and Other Organic Compounds
• Effect of Growth Phase on Stability of Fumarase Activity of Brevibacterium flavum Cells Immobilized with κ-Carrageenan

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