Stabilization of L-Aspartate β-Decarboxylase Activity of Pseudomonas dacunhae Immobilized with Carrageenan

概要

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To improve L-alanine production from L-aspartic acid by immobilized Pseudomonas dacunhae cells, the stabilization of L-aspartate β-decarboxylase activity of the immobilized cells was investigated.When P. dacunhae cells from the stationary growth-phase were used for immobilization, the operational stability of the enzyme activity of the cells immobilized with κ-carrageenan and treated with glutaraldehyde was found to be highest. When immobilized cells were treated with 0.4 M glutaraldehyde in the presence of 0.4-0.5 M L-lysine, a very stable immobilized preparation was obtained, the enzyme activity having a half-life of about 100 days at 37℃. Further, the activity at alkaline pH was improved by one-fourth over that at the optimum pH.
公益社団法人日本生物工学会の論文
1981-12-25